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https://hdl.handle.net/2440/129914
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Type: | Journal article |
Title: | Soybean CHX-type ion transport protein GmSALT3 confers leaf Na⁺ exclusion via a root derived mechanism, and Cl⁻ exclusion via a shoot derived process |
Other Titles: | Soybean CHX-type ion transport protein GmSALT3 confers leaf Na(+) exclusion via a root derived mechanism, and Cl(-) exclusion via a shoot derived process |
Author: | Qu, Y. Guan, R. Bose, J. Henderson, S.W. Wege, S. Qiu, L. Gilliham, M. |
Citation: | Plant, Cell and Environment, 2021; 44(3):856-869 |
Publisher: | Wiley |
Issue Date: | 2021 |
ISSN: | 0140-7791 1365-3040 |
Statement of Responsibility: | Yue Qu, Rongxia Guan, Jayakumar Bose, Sam W. Henderson, Stefanie Wege, Lijuan Qiu, Matthew Gilliham |
Abstract: | Soybean (Glycine max) yields are threatened by multiple stresses including soil salinity. GmSALT3 (a cation-proton exchanger protein) confers net shoot exclusion for both Na⁺ and Cl⁻ and improves salt tolerance of soybean; however, how the ER-localised GmSALT3 achieves this is unknown. Here, GmSALT3's function was investigated in heterologous systems and near-isogenic lines that contained the full-length GmSALT3 (NIL-T; salt-tolerant) or a truncated transcript Gmsalt3 (NIL-S; salt-sensitive). GmSALT3 restored growth of K⁺ -uptake-defective E. coli and contributed toward net influx and accumulation of Na⁺ , K⁺ , and Cl⁻ in Xenopus laevis oocytes, while Gmsalt3 was non-functional. Time-course analysis of NILs confirmed shoot Cl⁻ exclusion occurs distinctly from Na⁺ exclusion. Grafting showed that shoot Na⁺ exclusion occurs via a root xylem-based mechanism; in contrast, NIL-T plants exhibited significantly greater Cl⁻ content in both the stem xylem and phloem sap compared to NIL-S, indicating that shoot Cl⁻ exclusion likely depends upon novel phloem-based Cl⁻ recirculation. NIL-T shoots grafted on NIL-S roots contained low shoot Cl⁻ , which confirmed that Cl⁻ recirculation is dependent on the presence of GmSALT3 in shoots. Overall, these findings provide new insights on GmSALT3's impact on salinity tolerance and reveal a novel mechanism for shoot Cl⁻ exclusion in plants. |
Keywords: | Abiotic stress; CHX; ion transporter; salinity tolerance; shoot ion exclusion |
Rights: | © 2020 John Wiley & Sons Ltd |
DOI: | 10.1111/pce.13947 |
Grant ID: | http://purl.org/au-research/grants/arc/CE140100008 http://purl.org/au-research/grants/arc/FT130100709 http://purl.org/au-research/grants/arc/DE160100804 http://purl.org/au-research/grants/arc/DE170100346 |
Published version: | http://dx.doi.org/10.1111/pce.13947 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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