Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/134258
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Self-assembly of oriented antibody-decorated metal–organic framework nanocrystals for active-targeting applications |
Author: | Alt, K. Carraro, F. Jap, E. Linares-Moreau, M. Riccò, R. Righetto, M. Bogar, M. Amenitsch, H. Hashad, R.A. Doonan, C. Hagemeyer, C.E. Falcaro, P. |
Citation: | Advanced Materials, 2021; 34(21):2106607-1-2106607-7 |
Publisher: | Wiley |
Issue Date: | 2021 |
ISSN: | 0935-9648 1521-4095 |
Statement of Responsibility: | Karen Alt, Francesco Carraro, Edwina Jap, Mercedes Linares-Moreau, Raffaele Riccò, Marcello Righetto, Marco Bogar, Heinz Amenitsch, Rania A. Hashad, Christian Doonan, Christoph E. Hagemeyer, and Paolo Falcaro |
Abstract: | Antibody (Ab)-targeted nanoparticles are becoming increasingly important for precision medicine. By controlling the Ab orientation, targeting properties can be enhanced; however, to afford such an ordered configuration, cumbersome chemical functionalization protocols are usually required. This aspect limits the progress of Abs-nanoparticles toward nanomedicine translation. Herein, a novel one-step synthesis of oriented monoclonal Ab-decorated metal-organic framework (MOF) nanocrystals is presented. The crystallization of a zinc-based MOF, Zn₂(mIM₂(CO₃), from a solution of Zn²⁺ and 2-methylimidazole (mIM), is triggered by the fragment crystallizable (Fc) region of the Ab. This selective growth yields biocomposites with oriented Abs on the MOF nanocrystals (MOF*Ab): the Fc regions are partially inserted within the MOF surface and the antibody-binding regions protrude from the MOF surface toward the target. This ordered configuration imparts antibody-antigen recognition properties to the biocomposite and shows preserved target binding when compared to the parental antibodies. Next, the biosensing performance of the system is tested by loading MOF*Ab with luminescent quantum dots (QD). The targeting efficiency of the QD-containing MOF*Ab is again, fully preserved. The present work represents a simple self-assembly approach for the fabrication of antibody-decorated MOF nanocrystals with broad potential for sensing, diagnostic imaging, and targeted drug delivery. |
Keywords: | Antibodies; crystallization; metal–organic frameworks; self-assembly; targeting |
Description: | Vol. 34(21) pp 2106607-1 - 2106607-7 |
Rights: | © 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1002/adma.202106607 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1140465 http://purl.org/au-research/grants/nhmrc/GNT1154270 |
Published version: | http://dx.doi.org/10.1002/adma.202106607 |
Appears in Collections: | Chemical Engineering publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_134258.pdf | Published Version | 4.2 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.