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https://hdl.handle.net/2440/134633
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Type: | Journal article |
Title: | Translating n-3 polyunsaturated fatty acid status from whole blood to plasma and red blood cells during pregnancy: Translating n-3 status across blood fractions in pregnancy. |
Author: | Simmonds, L.A. Yelland, L.N. Best, K.P. Liu, G. Gibson, R.A. Makrides, M. |
Citation: | Prostaglandins, Leukotrienes and Essential Fatty Acids, 2021; 176:102367-102367 |
Publisher: | Elsevier |
Issue Date: | 2021 |
ISSN: | 0952-3278 1532-2823 |
Statement of Responsibility: | Lucy A Simmonds, Lisa N Yelland, Karen P Best, Ge Liu, Robert A Gibson, Maria Makrides |
Abstract: | Women with low n-3 (omega-3) status in pregnancy can reduce their risk of early preterm birth (<34 weeks' gestation) through n-3 long chain polyunsaturated fatty acid (LCPUFA) supplementation. As investigators measure fatty acid status in different blood fractions, equations are needed to compare results across studies. Similarly, derived cut-points for defining low and replete n-3 status are needed to assist clinical interpretation during early pregnancy. Our aims were to develop equations to convert the percentage of total n-3 fatty acids, EPA+DHA and DHA between whole blood, plasma and red blood cells (RBC), and to derive cut-points for defining low and replete total n-3 fatty acid status in plasma and RBC from those already established in whole blood. Using blood samples from 457 pregnant women in a multicentre randomised controlled trial, equations for these interconversions were developed using simple linear regression models. Measures of n-3 fatty acid status in whole blood and plasma were strongly related (R² > 0.85), while more moderate relationships were observed between measures in whole blood and RBC (R² 0.55 - 0.71), or plasma and RBC (R² 0.55 - 0.63). Using the conversion equations, established cut-points for low and replete n-3 status in whole blood (<4.2% and >4.9% of total fatty acids) converted to <3.7% and >4.3% of plasma total fatty acids, and to <7.3% and >8.1% of RBC total fatty acids. Agreement to define low and replete n-3 status was better between whole blood and plasma, rather than between whole blood and RBC. Our data also show that total n-3 fatty acids in plasma and serum are interchangeable. We conclude that either whole blood or plasma total n-3 fatty acids can be used to define low status in pregnancy and identify women who will most benefit from n-3 LCPUFA supplementation to reduce their risk of early birth. Further research is needed to determine the clinical utility of other fatty acid measures in various blood lipid fractions. |
Keywords: | Total n-3 fatty acids Docosahexaenoic acid Blood fraction conversions Pregnancy Early preterm birth |
Rights: | © 2021 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.plefa.2021.102367 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1135155 http://purl.org/au-research/grants/nhmrc/1061704 http://purl.org/au-research/grants/nhmrc/1046207 http://purl.org/au-research/grants/nhmrc/1052388 |
Published version: | http://dx.doi.org/10.1016/j.plefa.2021.102367 |
Appears in Collections: | Medicine publications |
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