Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/137150
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Type: | Journal article |
Title: | Single-nuclei and bulk-tissue gene-expression analysis of pheochromocytoma and paraganglioma links disease subtypes with tumor microenvironment |
Author: | Zethoven, M. Martelotto, L. Pattison, A. Bowen, B. Balachander, S. Flynn, A. Rossello, F.J. Hogg, A. Miller, J.A. Frysak, Z. Grimmond, S. Fishbein, L. Tischler, A.S. Gill, A.J. Hicks, R.J. Dahia, P.L.M. Clifton-Bligh, R. Pacak, K. Tothill, R.W. |
Citation: | Nature Communications, 2022; 13(1):6262-6262 |
Publisher: | Springer Science and Business Media LLC |
Issue Date: | 2022 |
ISSN: | 2041-1723 2041-1723 |
Statement of Responsibility: | Magnus Zethoven, Luciano Martelotto, Andrew Pattison, Blake Bowen, Shiva Balachander, Aidan Flynn, Fernando J. Rossello, Annette Hogg, Julie A.Miller, Zdenek Frysak, Sean Grimmond, Lauren Fishbein, Arthur S. Tischler, Anthony J. Gill, Rodney J. Hicks, Patricia L. M. Dahia, Roderick Clifton-Bligh, Karel Pacak, Richard W. Tothill |
Abstract: | Pheochromocytomas (PC) and paragangliomas (PG) are rare neuroendocrine tumors associated with autonomic nerves. Here we use single-nuclei RNA-seq and bulk-tissue gene-expression data to characterize the cellular composition of PCPG and normal adrenal tissues, refine tumor gene-expression subtypes and make clinical and genotypic associations. We confirm seven PCPG gene-expression subtypes with significant genotype and clinical associations. Tumors with mutations in VHL, SDH-encoding genes (SDHx) or MAML3-fusions are characterized by hypoxia-inducible factor signaling and neoangiogenesis. PCPG have few infiltrating lymphocytes but abundant macrophages. While neoplastic cells transcriptionally resemble mature chromaffin cells, early chromaffin and neuroblast markers are also features of some PCPG subtypes. The gene-expression profile of metastatic SDHx-related PCPG indicates these tumors have elevated cellular proliferation and a lower number of non-neoplastic Schwann-cell-like cells, while GPR139 is a potential theranostic target. Our findings therefore clarify the diverse transcriptional programs and cellular composition of PCPG and identify biomarkers of potential clinical significance. |
Keywords: | Humans Paraganglioma Pheochromocytoma Adrenal Gland Neoplasms Succinate Dehydrogenase Tumor Microenvironment |
Rights: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. |
DOI: | 10.1038/s41467-022-34011-3 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1108032 |
Published version: | http://dx.doi.org/10.1038/s41467-022-34011-3 |
Appears in Collections: | Medical Sciences publications |
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hdl_137150.pdf | Published version | 5.84 MB | Adobe PDF | View/Open |
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