Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/92412
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Type: | Journal article |
Title: | MALDI imaging mass spectrometry of N-linked glycans on formalin-fixed paraffin-embedded murine kidney |
Author: | Gustafsson, O. Briggs, M. Condina, M. Winderbaum, L. Pelzing, M. McColl, S. Everest-Dass, A. Packer, N. Hoffmann, P. |
Citation: | Analytical and Bioanalytical Chemistry, 2015; 407(8):2127-2139 |
Publisher: | Springer-Verlag |
Issue Date: | 2015 |
ISSN: | 1618-2642 1618-2650 |
Statement of Responsibility: | Ove J. R. Gustafsson, Matthew T. Briggs, Mark R. Condina, Lyron J. Winderbaum, Matthias Pelzing, Shaun R. McColl, Arun V. Everest-Dass, Nicolle H. Packer, Peter Hoffmann |
Abstract: | Recent developments in spatial proteomics have paved the way for retrospective in situ mass spectrometry (MS) analyses of formalin-fixed paraffin-embedded clinical tissue samples. This type of analysis is commonly referred to as matrix-assisted laser desorption/ionization (MALDI) imaging. Recently, formalin-fixed paraffin-embedded MALDI imaging analyses were augmented to allow in situ analyses of tissue-specific N-glycosylation profiles. In the present study, we outline an improved automated sample preparation method for N-glycan MALDI imaging, which uses in situ PNGase F-mediated release and measurement of N-linked glycans from sections of formalin-fixed murine kidney. The sum of the presented data indicated that N-glycans can be cleaved from proteins within formalin-fixed tissue and characterized using three strategies: (i) extraction and composition analysis through on-target MALDI MS and liquid chromatography coupled to electrospray ionization ion trap MS; (ii) MALDI profiling, where N-glycans are released and measured from large droplet arrays in situ; and (iii) MALDI imaging, which maps the tissue specificity of N-glycans at a higher resolution. Thus, we present a complete, straightforward method that combines MALDI imaging and characterization of tissue-specific N-glycans and complements existing strategies. |
Keywords: | MALDI imaging; MALDI; Mass spectrometry; Glycans; N-linked |
Rights: | © The Author(s) 2014. This article is published with open access at Springerlink.com |
DOI: | 10.1007/s00216-014-8293-7 |
Grant ID: | http://purl.org/au-research/grants/arc/LP110100693 |
Published version: | http://dx.doi.org/10.1007/s00216-014-8293-7 |
Appears in Collections: | Aurora harvest 7 IPAS publications |
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hdl_92412.pdf | Published version | 4.13 MB | Adobe PDF | View/Open |
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