Isolation, molecular characterization and location of telomeric sequences of the basidiomycete Pleurotus ostreatus var. florida
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
The white rot fungus Pleurotus ostreatus is an edible basidiomycete of increasing
biotechnological interest due to its ability to degrade both wood and
chemicals related to lignin degradation products.
Telomeres are specialized structures at the end of all eukaryotic chromosomes.
Ensure chromosome stability and protect the ends from degradation
and from fusing with other chromosomes. Telomer ...
[++]
The white rot fungus Pleurotus ostreatus is an edible basidiomycete of increasing
biotechnological interest due to its ability to degrade both wood and
chemicals related to lignin degradation products.
Telomeres are specialized structures at the end of all eukaryotic chromosomes.
Ensure chromosome stability and protect the ends from degradation
and from fusing with other chromosomes. Telomeres sequences are extraordinary
highly conserved in evolution. The loss of telomeric repeats triggers
replicative senescence in cells.
For identification of restriction telomeric fragments in a previously described
linkage map of Pleurotus ostreatus var. florida (Larraya et al., 2000),
dikaryotic and eighty monokaryotic genomic DNAs were digested with
diferents restriction enzymes (BamHI, BglII, HindIII, EcoRI, PstI, SalI, XbaI
and XhoI) electrophoresed and transferred to nylon membranes. Numerous
polymorphic bands were observed when membranes were hibridized with
human telomericd probe (TTAGGG)132 (heterologous probe).
Telomeric restriction fragments were genetically mapped to a previously described
linkage map of Pleurotus ostreatus var.florida, using RFLPs identified by
a human telomeric probe (tandemly repeating TTAGGG hexanucleotide).
Segregation of each telomeric restriction fragment was recorded as the presence
vs. absence of a hibridizing band. Segregation data for seventy three telomeric
restriction fragments was used as an input table to be analysed as described
by Ritter et al. (1990) and by Ritter and Salamini (1996) by using the MAPRF
program software. Seventeen out of twenty two telomeres were identified.
Telomere and telomere-associated (TA) DNA sequences of the basidiomycete
Pleurotus ostreatus were isolated by using a modified version of single-
specific-primer polymerase chain reaction (SSP-PCR) technique (Sohapal
et al., 2000). Telomeres of Pleurotus ostreatus contain at least twenty five
copies of non-coding tandemly repeated sequence (TTAGGG). [--]
Materias
Pleurotus ostreatus var. Florida,
Telomeres
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila