Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population
Fecha
2013Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
10.1371/journal.pone.0077271
Resumen
A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture
of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 plaque purified
virus were characterized. Ten distinct genotypes were identified (named A through J). SfCOL-A was the most
prevalent (71±2%; mean ± SE) showing a PstI restriction ...
[++]
A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture
of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 plaque purified
virus were characterized. Ten distinct genotypes were identified (named A through J). SfCOL-A was the most
prevalent (71±2%; mean ± SE) showing a PstI restriction profile indistinguishable to that of SfCOL-wt. The remaining
nine genotypes presented genomic deletions of 3.8 - 21.8 Kb located mainly between nucleotides 11,436 and 33,883
in the reference genome SfMNPV-B, affecting the region between open reading frames (ORFs) sf20 and sf33. The
insecticidal activity of each genotype from SfCOL-wt and several mixtures of genotypes was compared to that of
SfCOL-wt. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the
speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than
SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of genotype mixtures cooccluded
within OBs were consistently reduced in two-genotype mixtures involving equal proportions of SfCOL-A and
one of three deletion genotypes (SfCOL-C, -D or -F). Speed of kill and OB production were improved only when the
certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any
of the component genotypes, or mixtures thereof. Deleted genotypes reduced OB potency but increased OB
production of the SfCOL-wt population, which is structured to maximize the production of OBs in each infected host. [--]
Materias
Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV),
Insecticidial properties,
Deletion genotypes
Editor
Public Library of Science
Publicado en
Plos One, 8(10): e77271
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila /
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study received financial support from CSIC–COLCIENCIAS Cooperation project 2008CO0029 and the Colombian Ministry of Agriculture and Rural Development (Project 007X4648-176-968).