Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs
Fecha
2012Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1128/jcm.02099-12
Resumen
The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for
the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic
sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations
with differen ...
[++]
The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for
the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic
sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations
with different Salmonella prevalences (14 farms with a ≥20% prevalence and 13 farms with a<20% prevalence) and
through the use of latent-class models in concert with Bayesian inference, assuming 100% ISO specificity, and an invA-based
PCR as the second diagnostic method. The SeISO was estimated to be close to 87%, while the sensitivity of the PCR reached up to
83.6% and its specificity was 97.4%. Interestingly, the bacteriological reanalysis of 33 potential false-negative (PCR-positive)
samples allowed isolation of 19 (57.5%) new Salmonella strains, improving the overall diagnostic accuracy of the bacteriology.
Considering the usual limitations of bacteriology regarding Se, these results support the adequacy of the ISO for the detection of
Salmonella spp. from MLN and also that of the PCR-based method as an alternative or complementary (screening) test for the
diagnosis of pig salmonellosis, particularly considering the cost and time benefits of the molecular procedure. [--]
Materias
ISO 6579:2002/Amd 1:2007,
Salmonella spp.,
Mesenteric lymph nodes,
Pigs,
Salmonellosis
Editor
American Society for Microbiology
Publicado en
Journal of Clinical Microbiology, January 2013 Volume 51 Number 1, p. 89–94
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This study was funded by the Instituto Nacional de Investigación y Tecnología
Agraria y Alimentaria (INIA) of Spain (research grants no.
RTA2007-65 and FAU2008-16) and the Gobierno de Navarra (project
reference IIQ14064.RI1). A fellowship for V.G. was funded by the Public
University of Navarre, and B.S.R. received a contract from CSIC (JAEDoc
program).