Fluorescence Study of Riboflavin Interactions with Graphene Dispersed in Bioactive Tannic Acid
Authors
San Andrés Lledó, María PazIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/49651DOI: 10.3390/ijms22105270
ISSN: 1422-0067
Date
2021-05-17Academic Departments
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Teaching unit
Unidad docente Química Analítica e Ingeniería Quimica
Funders
Ministerio de Ciencia, Innovación y Universidades
Bibliographic citation
International Journal of Molecular Sciences, 2021, v. 22, n. 10, p. 5270
Keywords
tannic acid
Graphene
fluorescence
quenching
riboflavin
Project
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-093375-B-I00/ES/Funcionalización de grafeno y sus derivados con biotensioactivos y compuestos bioactivos como nueva herramienta para la determinación de compuestos de interés biológico
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
The potential of tannic acid (TA) as a dispersing agent for graphene (G) in aqueous solutions and its interaction with riboflavin have been studied under different experimental conditions. TA induces quenching of riboflavin fluorescence, and the effect is stronger with increasing TA concentration, due to pi-pi interactions through the aromatic rings, and hydrogen bonding interactions between the hydroxyl moieties of both compounds. The influence of TA concentration, the pH, and the G/TA weight ratio on the quenching magnitude, have been studied. At a pH of 4.1, G dispersed in TA hardly influences the riboflavin fluorescence, while at a pH of 7.1, the nanomaterial interacts with riboflavin, causing an additional quenching to that produced by TA. When TA concentration is kept constant, quenching of G on riboflavin fluorescence depends on both the G/TA weight ratio and the TA concentration. The fluorescence attenuation is stronger for dispersions with the lowest G/TA ratios, since TA is the main contributor to the quenching effect. Data obey the Stern-Volmer relationship up to TA 2.0 g L-1 and G 20 mg L-1. Results demonstrate that TA is an effective dispersant for graphene-based nanomaterials in liquid medium and a green alternative to conventional surfactants and synthetic polymers for the determination of biomolecules.
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Fluorescence_SanAndres_IntJMol ... | 4.424Mb |
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