Different roles of Nrf2 and NFkappaB in the antioxidant imbalance produced by esculetin or quercetin on NB4 leukemia cells
Authors
Rubio Serrano, Virginia; García Pérez, Ana Isabel; Herráez Sánchez, Ángel; Díez Ballesteros, José CarlosIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/59516Related data: https://doi.org/10.21950/MMPB5K
DOI: 10.1016/j.cbi.2018.08.015
ISSN: 0009-2797
Date
2018-08-30Bibliographic citation
Chemico-Biological Interactions, 2018, v. 294, n. , p. 158-166
Keywords
Antioxidants
Apoptosis
Esculetin
Hydrogen peroxide
Reactive oxygen species
Tert-butylhydroperoxide
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© 2018 Elsevier B.V.
Access rights
info:eu-repo/semantics/openAccess
Abstract
Esculetin (6,7-dihydrocoumarin) and the ?avonoid quercetin (3,5,7,3?,4? pentahydroxy?avone) are compounds that could change the balance of redox homeostasis. NB4 leukemia cells were treated with 25 ?M quercetin for 24 h and with esculetin at either 100 or 500 ?M for di?erent times. Quercetin increased the levels of proin?ammatory NFkB p65 in the nucleus correspondingly reducing them in the cytosol. The levels of NFkB p65 decreased in the nucleus at high esculetin concentration treatments for long times (19 h), concomitantly increasing the levels of anti-in?ammatory NFkB p50 in the nucleus. This could suggest formation of inhibitory p50 homodimers possibly related with anti-in?ammatory response. Lipoxygenase expression was reduced either by esculetin or quercetin. A signi?cant increase of Nrf2 in the nucleus of NB4 cells treated with 100 ?M esculetin for 19 h was observed. Quercetin increased the levels of Nrf2 in the cytosol reducing them in the nucleus. Superoxide dismutase expression increased in NB4 cells treated with esculetin in contrast with quercetin. All these data support a relevant di?erential role for NFkB and Nrf2 in anti-in?ammatory and redox response when apoptosis was induced by esculetin or quercetin in human leukemia NB4 cells.
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