Antibacterial biopolymer gel coating on meshes used for abdominal hernia repair promotes effective wound repair in the presence of infection
Authors
Benito Martínez, Selma; Pérez Köhler, Bárbara; Rodríguez Mancheño, Marta; García-Moreno Nisa, Francisca; Gómez Gil, Verónica; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59596DOI: 10.3390/polym13142371
ISSN: 2073-4360
Date
2021Affiliation
Universidad de Alcalá. Departamento de Cirugía, Ciencias Médicas y Sociales; Universidad de Alcalá. Departamento de Medicina y Especialidades MédicasFunders
Ministerio de Ciencia e Innovación
Bibliographic citation
Polymers, 2021, v. 13, n. 14, p. 2371-
Keywords
Antimicrobial
biopolymers
chlorhexidine
hernia
mesh coating
mesh infection
rifampicin
tissue repair
Description / Notes
14 p.
Project
info:eu-repo/grantAgreement/MICIN//SAF2017-89481-P/ES//
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© 2021 by the author (s)
Attribution 4.0 International (CC BY 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall tissue repair of coating meshes with a chlorhexidine or rifampicin-carboxymethylcellulose biopolymer gel in a Staphylococcus aureus (S. aureus) infection model. Partial abdominal wall defects were created in New Zealand white rabbits (n = 20). Four study groups were established according to whether the meshes were coated or not with each of the antibacterial gels. Three groups were inoculated with S. aureus and finally repaired with lightweight polypropylene mesh. Fourteen days after surgery, implanted meshes were recovered for analysis of the gene and protein expression of collagens, macrophage phenotypes, and mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Compared to uncoated meshes, those coated with either biopolymer gel showed higher collagen 1/3 messenger RNA and collagen I protein expression, relatively increased VEGF mRNA expression, a significantly reduced macrophage response, and lower relative amounts of MMPs mRNAs. Our findings suggest that following mesh implant these coatings may help improving abdominal wall tissue repair in the presence of infection
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