Gold nanoparticles coated with carbosilane dendrons in protein sample preparation
Authors
Vásquez Villanueva, Romy Ángela; Peña González, Cornelia Emeritina; Sánchez-Nieves Fernández, Javier; Mata de la Mata, Francisco Javier de la; Marina Alegre, María Luisa; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59695DOI: doi.org/10.1007/s00604-019-3587-2
ISSN: 1436-5073
Date
2019-07-04Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaFunders
Ministerio de Economía, Comercio y Empresa
Universidad de Alcalá
Centro de investigación Biomédica en Red. Bioingeniería, Biomateriales y Nanomedicina
Bibliographic citation
Microchimica Acta , 2019, v. 186, n. 8, p. 507-516
Keywords
Dendrimer
Interaction
Protein extraction
Nanomaterial
Sustainability
Project
info:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/
info:eu-repo/grantAgreement/MINECO//Q2017-86224-P/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Springer-Verlag
Access rights
info:eu-repo/semantics/openAccess
Abstract
The feasibility of using carbosilane dendronized gold nanoparticles (GNPs) for protein sample preparation was evaluated. Three different dendrons with three different generations (1G, 2G, and 3G) were employed to modify the GNPs, viz. sulfonate terminated (STC-GNPs), carboxylate terminated (CTC-GNPs), and trimethylammonium terminated (ATC-GNPs)dendrons. The synthesis of the CTC-GNP is described. The other dendronized GNPs were synthesized using previously described routes. Bovine serum albumin, lysozyme, and myoglobin were employed to study the potential of GNPs to interact with proteins. The interaction between the GNPs and the proteins was evaluated using fluorescence spectroscopy and polyacrylamide gel electrophoresis. The CTC-GNPs and STC-GNPs under acidic and neutral conditions, respectively, promoted the establishment of electrostatic interactions with positively charged proteins. Proteins from 10 to 75kDa molecular weights interacted with GNPs at protein: nanoparticle ratios of 1:0.25. The GNPs were applied to the extraction of proteins from a peach seed. In the authors' perception, the method is a clean alternative to established extraction methods based on the use of organic or polluting chemicals.
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Gold_Vasquez_MicrochimActa_2019.pdf | 1.541Mb |
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