Identification of a novel 2-oxindole fluorinated derivative as in vivo antitumor agent for prostate cancer acting via AMPK activation
Authors
Bort Bueno, Alicia Carmen; Quesada Sánchez, Sergio; Ramos Torres, Ágata; Gargantilla, Marta; Priego, Eva Maria; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/60111DOI: 10.1038/s41598-018-22690-2
ISSN: 2045-2322
Date
2018-03-12Funders
Ministerio de Economía y Competitividad
Junta de Comunidades de Castilla-La Mancha
Fondo Europeo de Desarrollo Regional
Comunidad Autónoma de Madrid
Fundación Tatiana Pérez de Guzmán el Bueno
Junta para la Ampliacion de Estudios
Bibliographic citation
Scientific Reports, 2018, v. 8, n. 1 (4370), p. 1-18
Project
info:eu-repo/grantAgreement/MINECO//BFU201231444/ES/
info:eu-repo/grantAgreement/MINECO//CTQ2010-19690/ES/
info:eu-repo/grantAgreement/JCCM//POII11-0159-0054/ES/
info:eu-repo/grantAgreement/CAM// S2010-BMD2308/ES/
info:eu-repo/grantAgreement/FT//2013-001/ES/
info:eu-repo/grantAgreement/JAE//JAE-Pre-2011/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The Author(s) 2018
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
The key metabolic sensor adenosine monophosphate-dependent kinase (AMPK) has emerged as a promising therapeutic target for cancer prevention and treatment. Besides its role in energy homeostasis, AMPK blocks cell cycle, regulates autophagy and suppresses the anabolic processes required for rapid cell growth. AMPK is especially relevant in prostate cancer in which activation of lipogenic pathways correlate with tumor progression and aggressiveness. This study reports the discovery of a new series of 2-oxindole derivatives whose AMPK modulatory ability, as well as the antitumoral profile in prostate cancer cells, was evaluated. One of the assayed compounds, compound 8c, notably activated AMPK in cultured PC-3, DU145 and LNCaP prostate cancer cells. Likewise, compound 8c caused PC-3, DU145 and LNCaP cells viability inhibition. Selective knocking down of ?1 or ?2 isoforms as well as in vitro assays using human recombinant ?1?1?1 or ?2?1?1 AMPK isoforms revealed that compound 8c exhibit preference for AMPK?1. Consistent with efficacy at the cellular level, compound 8c was potent in suppressing the growth of PC-3 xenograft tumors. In conclusion, our results show that a new 2-oxindole fluorinated derivative exerts potent in vivo antitumor actions against prostate cancer cells, indicating a promising clinical therapeutic strategy for the treatment of androgen-independent prostate cancer.
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Identification_Bort_SciRep_2018.pdf | 3.803Mb |
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