Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions
Authors
Sánchez-Nieves Fernández, JavierIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/60444DOI: 10.1016/j.colsurfb.2017.12.009
ISSN: 1873-4367
Date
2018-02Academic Departments
Universidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica
Teaching unit
Unidad docente Química Inorgánica
Funders
University of Alcalá; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN); Instituto de Investigación Sanitaria Gregorio Marañón; Universitat de Barcelona
Bibliographic citation
Coll. Surf. B Biointerfaces, 2018, v. 162, p. 380-388
Keywords
Dendrimers
Carbosilane
Gene therapysi
RNA
Molecular dynamics
Force spectroscopy
Project
CTQ-2014-54004-P (MINECO); RD12/0017/0037 (ISCIII); RD16/0025/0019 (ISCIII); PI16/01863 (FIS); PI14/00882 (FIS); CYTED 214RT0482; FIS2016-80458-P (MINECO); 15-05903S (Czech Science); LM2015073 (Czech Republic)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
The interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane dendrimers of generations 1-3 with two different ammonium functions at the periphery ([-NMe2R](+), R=Me, (CH2)(2)OH) has been studied by experimental techniques (zeta potential, electrophoresis, single molecule pulling experiments) and molecular dynamic calculations. These studies state the presence of different forces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higher dendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compounds can penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the particular case of first generation dendrimer [G(1)O(3)(NMe2(CH2)(2)OH))(6)](6+); the presence of hydroxyl groups reinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers do not cover the RNA, only higher generation derivatives protect RNA from degradation.
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Study_Sanchez_CollSurfB_2018.pdf | 1.102Mb |
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