Characterization of HIV type 1 genetic diversity among South African participants enrolled in the AIDS Vaccine Integrated Project (AVIP) Study
Date
2010
Authors
Papathanasopoulos M.A.
Vardas E.
Wallis C.
Glashoff R.
Butto S.
Poli G.
Malnati M.
Clerici M.
Ensoli B.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The genetic diversity of HIV-1 strains circulating among HIV-1-infected South Africans was investigated in a cohort of 420 individuals enrolled as part of the AIDS Vaccine Integrated Project (AVIP) study. Representative samples (10%) were randomly selected from treatment-naive participants. Viral RNA was extracted for reverse transcriptase-initiated amplification and population-based sequencing of partial pol (encompassing protease and reverse transcriptase) and full-length integrase. Overall, HIV-1 sequences confirmed that 97.1% and 96.9% were HIV-1 subtype C in pol and integrase, respectively. Two participants were infected with unique A1/C and C/A1 recombinants in pol/integrase. Further pol sequence analysis identified mutation patterns associated with high level resistance to NNRTIs in two participants, whereas no primary mutations conferring resistance to integrase inhibitors were detected. The predominance of HIV-1 subtype C in South African populations is therefore confirmed in the AVIP cohort finalized for testing preventive or therapeutic vaccines against HIV-1 infection. © 2010, Mary Ann Liebert, Inc.
Description
Keywords
etravirine, integrase, integrase inhibitor, nonnucleoside reverse transcriptase inhibitor, Pol protein, proteinase, virus RNA, adult, article, CD4 lymphocyte count, female, gene mutation, gene sequence, genetic variability, human, Human immunodeficiency virus 1, Human immunodeficiency virus 1 infection, major clinical study, male, Negro, nonhuman, nucleotide sequence, phylogenetic tree, priority journal, reverse transcription polymerase chain reaction, South Africa, Adult, Cluster Analysis, Female, Genetic Variation, Genotype, HIV Infections, HIV-1, Humans, Male, Middle Aged, Molecular Sequence Data, pol Gene Products, Human Immunodeficiency Virus, RNA, Viral, Sequence Analysis, DNA, Sequence Homology, South Africa
Citation
AIDS Research and Human Retroviruses
26
6
26
6