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  5. Development of a bacterial propionate-biosensor for anaerobic digestion monitoring
 
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Development of a bacterial propionate-biosensor for anaerobic digestion monitoring

Author(s)
Sweeney, Joseph  
Murphy, Cormac D.  
McDonnell, Kevin  
Uri
http://hdl.handle.net/10197/10829
Date Issued
2018-02-01
Date Available
2019-07-01T13:46:43Z
Abstract
Monitoring anaerobic digestion (AD) leachate for changes in acetate and propionate concentrations is essential for effective AD operation. In this paper the development of a novel propionate cell-based biosensor is described. A previously designed E. coli mutant (IMD Wldgy) that could selectively determine acetate concentrations in synthetic leachates, based on oxygen uptake measurements, was used as a starting point in the development of a propionate biosensor. However, the propionate-grown IMD Wldgy cells exhibited extremely low propionate:acetate O2 consumption ratios (1:2.4). Screening for alternative propionate-grown E. coli strains naturally possessing a more favourable propionate:acetate O2 consumption ratio identified strain IMD 1, which exhibited a positive ratio (1.6:1). To improve the selectivity of the strain, successive gene knockouts were performed generating the IMD 1 hldgyep mutant. However, propionate-grown IMD 1hdlgyep's O2 consumption ratio was deemed too low to be considered as a propionate detecting bio-element. It was reasoned that the mechanisms by which E. coli activates acetate had to be removed. Deleting acs (acetyl-CoA synthesase) and ackA (acetate kinase) from IMD Wldgyep, resulted in an E. coli IMD Wldgyepak knockout mutant that, when grown on propionate, produced a mean propionate:acetate O2 consumption ratio of approx. 13:1. The resulting IMD Wldgyep and IMD Wldgyepak strains, which formed the acetate- and propionate-biosensor, respectively, were capable of detecting acetate and propionate concentrations ranging from 0.05 mM to 4.5 mM within two-phase AD synthetic leachates.
Sponsorship
Science Foundation Ireland
Other Sponsorship
Charles Parsons Energy Research Programme
Type of Material
Journal Article
Publisher
Elsevier BV
Journal
Enzyme and Microbial Technology
Volume
109
Start Page
51
End Page
57
Copyright (Published Version)
2017 Elsevier
Subjects

Escherichia coli

Oxygen

Acetates

Acs

Biosensor

Anaerobic digestion

Digestion

Mutation

AckA

VFA

Monitoring and contro...

Synthetic leachate

DOI
10.1016/j.enzmictec.2017.09.011
Language
English
Status of Item
Peer reviewed
ISSN
0141-0229
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
File(s)
No Thumbnail Available
Name

REVISION Propionate biosensor EMT CLEAN.docx

Size

310.66 KB

Format

Unknown

Checksum (MD5)

a382147d1d65e57988dbf93a33a3c67d

Owning collection
Biomolecular and Biomedical Science Research Collection
Mapped collections
Institute of Food and Health Research Collection

Item descriptive metadata is released under a CC-0 (public domain) license: https://creativecommons.org/public-domain/cc0/.
All other content is subject to copyright.

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