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  5. Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate
 
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Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate

Author(s)
Sweeney, Joseph  
Murphy, Cormac D.  
McDonnell, Kevin  
Uri
http://hdl.handle.net/10197/7963
Date Issued
2015-12
Date Available
2016-09-19T14:45:26Z
Abstract
Development of a biosensor for the convenient measurement of acetate and propionate concentrations in a two-phase anaerobic digestor (AD) requires a bacterium that will be unresponsive to the other organic acids present in the leachate, of which lactate is the most abundant. Successive gene knockouts of E.coli W3110 d-lactate dehydrogenase (dld), l-lactate dehydrogenase (lldD), glycolate oxidase (glcD) and a suspected l-lactate dehdrogenase (ykgF) were performed. The resulting quadruple mutant (IMD Wldgy) was incapable of growth on d- and l-lactate, whereas the wild type grew readily on these substrates. Furthermore, the O2 consumption rates of acetate-grown IMD Wldgy cell suspensions supplied with either acetate (0.1 mM) or a synthetic leachate including acetate (0.1 mM) and dl-lactate (1 mM) were identical (2.79 and 2.70 mg l−1 min−1, respectively). This was in marked contrast to similar experiments with the wild type which gave initial O2 consumption rates of 2.00, 2.36 and 2.97 mg l−1 min−1 when cell suspensions were supplied with acetate (0.1 mM), acetate (0.1 mM) plus d-lactate (1 mM) or acetate (0.1 mM) plus l-lactate (1 mM), respectively. The knockout strain provides a platform for the design of a biosensor that can accessibly monitor acetate and propionate concentrations in AD leachate via O2-uptake measurements.
Sponsorship
Science Foundation Ireland
Other Sponsorship
Charles Parsons Energy Research Programme
Type of Material
Journal Article
Publisher
Springer
Journal
Applied Microbiology and Biotechnology
Volume
99
Issue
23
Start Page
10209
End Page
10214
Copyright (Published Version)
2015 Springer-Verlag Berlin Heidelberg
Subjects

Anaerobic digestion

Biosensor

Volatile fatty acids

Synthetic leachate

DOI
10.1007/s00253-015-6887-4
Language
English
Status of Item
Peer reviewed
This item is made available under a Creative Commons License
https://creativecommons.org/licenses/by-nc-nd/3.0/ie/
File(s)
No Thumbnail Available
Name

Sweeney_et_al_Revision_July_2015.docx

Size

229.39 KB

Format

Microsoft Word

Checksum (MD5)

3a3e2e8c2ff5ff2838678783310798d8

Owning collection
Biomolecular and Biomedical Science Research Collection
Mapped collections
Biosystems and Food Engineering Research Collection•
Institute of Food and Health Research Collection

Item descriptive metadata is released under a CC-0 (public domain) license: https://creativecommons.org/public-domain/cc0/.
All other content is subject to copyright.

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