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Título: | The identification of wadB, a new glycosyltransferase gene, confirms the branched structure and the role in virulence of the lipopolysaccharide core of Brucella abortus |
Autor: | Gil Ramírez, Yolanda CSIC; Conde Álvarez, Raquel; Palacios Chaves, Leyre CSIC ; Zúñiga Ripa, Amaia; Grilló, María Jesús CSIC ORCID ; Arce Gorvel, Vilma; Hanniffy, Sean; Moriyón, Ignacio; Iriarte, Maite | Palabras clave: | wadC wadB Virulence Brucella Glycosyltransferase Lipopolysaccharide |
Fecha de publicación: | ago-2014 | Editor: | Elsevier | Citación: | Microbial Pathogenesis 73: 53-59 (2014) | Resumen: | Brucellosis is a worldwide extended zoonosis caused by Brucella spp. These gram-negative bacteria are not readily detected by innate immunity, a virulence-related property largely linked to their surface lipopolysaccharide (LPS). The role of the LPS lipid A and O-polysaccharide in virulence is well known. Moreover, mutation of the glycosyltransferase gene wadC of Brucella abortus, although not affecting O-polysaccharide assembly onto the lipid-A core section causes a core oligosaccharide defect that increases recognition by innate immunity. Here, we report on a second gene ( wadB) encoding a LPS core glycosyltransferase not involved in the assembly of the O-polysaccharide-linked core section. As compared to wild-type B.abortus, a wadB mutant was sensitive to bactericidal peptides and non-immune serum, and was attenuated in mice and dendritic cells. These observations show that as WadC, WadB is also involved in the assembly of a branch of Brucella LPS core and support the concept that this LPS section is a virulence-related structure. © 2014 Elsevier Ltd. | URI: | http://hdl.handle.net/10261/100898 | DOI: | 10.1016/j.micpath.2014.06.002 | Identificadores: | doi: 10.1016/j.micpath.2014.06.002 issn: 1096-1208 |
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