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Título: | Fission yeast cell cycle synchronization methods |
Autor: | Tormos-Pérez, Marta CSIC; Pérez-Hidalgo, Livia CSIC ORCID; Moreno, Sergio CSIC ORCID | Palabras clave: | Centrifugal elutriation Block and release G1-phase Schizosaccharomyces pombe Fission yeast Lactose gradient Mitosis S-phase G2-phase Synchronization |
Fecha de publicación: | 2016 | Editor: | Springer Nature | Citación: | Yeast Cytokinesis. Methods and Protocols: 293-308 (2016) | Serie: | Methods in Molecular Biology 1369 | Resumen: | Fission yeast cells can be synchronized by cell cycle arrest and release or by size selection. Cell cycle arrest synchronization is based on the block and release of temperature-sensitive cell cycle mutants or treatment with drugs. The most widely used approaches are cdc10-129 for G1; hydroxyurea (HU) for early S-phase; cdc25-22 for G2, and nda3-KM311 for mitosis. Cells can also be synchronized by size selection using centrifugal elutriation or a lactose gradient. Here we describe the methods most commonly used to synchronize fission yeast cells. | URI: | http://hdl.handle.net/10261/157500 | Identificadores: | isbn: 978-1-4939-3144-6 |
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