Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/169487
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | An enzymatic system for decolorization of wastewater dyes using immobilized CueO laccase-like multicopper oxidase on poly-3-hydroxybutyrate |
Autor: | Bello-Gil, Daniel; Roig-Molina, Emma; Fonseca, Jennifer; Sarmiento-Ferrández, María Dolores; Ferrándiz-García, Marcela; Franco, Esther; Mira, Elena; Maestro, Beatriz CSIC ORCID; Sanz, Jesús M. CSIC ORCID CVN | Palabras clave: | High-level expression Escherichia-coli Bacterial laccase Cuprous oxidase RNA-polymerase Cloned genes Proteins Enzymes Polyhydroxyalkanoates Industrial |
Fecha de publicación: | 11-sep-2018 | Editor: | John Wiley & Sons | Citación: | Microbial Biotechnology 11(5) 881–892 (2018) | Resumen: | The presence of synthetic dyes in wastewaters generated by the textile industry constitutes a serious environmental and health problem that urges the scientific community on an appropriate action. As a proof-of-concept, we have developed a novel approach to design enzymatic bioreactors with the ability to decolorize dye solutions through the immobilization of the bacterial CueO laccase-like multicopper oxidase from Escherichia coli on polyhydroxybutyrate (PHB) beads by making use of the BioF affinity tag. The decolorization efficiency of the system was characterized by a series of parameters, namely maximum enzyme adsorption capacity, pH profile, kinetic constants, substrate range, temperature and bioreactor recycling. Depending on the tested dye, immobilization increased the catalytic activity of CueO by up to 40-fold with respect to the soluble enzyme, reaching decolorization efficiencies of 45-90%. Our results indicate that oxidase bioreactors based on polyhydroxyalkanoates are a promising alternative for the treatment of coloured industrial wastewaters. | Descripción: | 12 p.-6 fig.-2 tab. | Versión del editor: | https://doi.org/10.1111/1751-7915.13287 | URI: | http://hdl.handle.net/10261/169487 | DOI: | 10.1111/1751-7915.13287 | E-ISSN: | 1751-7915 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Bello-Gil_et_al-2018-Microbial_Biotechnology.pdf | Artículo principal | 646,42 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
10
checked on 07-abr-2024
SCOPUSTM
Citations
28
checked on 25-abr-2024
WEB OF SCIENCETM
Citations
20
checked on 26-feb-2024
Page view(s)
382
checked on 22-abr-2024
Download(s)
298
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.