Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/197531
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage |
Autor: | Martínez, Rubén CSIC ORCID ; Navarro-Martín, Laia CSIC ORCID ; van Antro, Morgane; Fuertes, Inmaculada CSIC ORCID; Casado, Marta CSIC ORCID ; Barata Martí, Carlos CSIC ORCID ; Piña, Benjamín CSIC ORCID | Palabras clave: | Mass spectrometry Adverse outcome pathway Endocrine disruption Phenols Thin layer chromatography Mode of action Yolk sac malabsorption |
Fecha de publicación: | 20-may-2020 | Editor: | Elsevier | Citación: | Chemosphere 246: 125704 (2020) | Resumen: | Bisphenol A (BPA; 4,4’-(propane-2,2-diyl)diphenol) has been shown to act as an obesogen and to disrupt lipid metabolism in zebrafish eleutheroembryos (ZE). To characterize the consequences of this disruption, we performed a detailed lipidomic study using ZE exposed to different BPA concentrations (0, 4, 6 and 8 mg/L of BPA) from day 2 to up to day 6 post fertilization (dpf). Total lipids at 4, 5 and 6 dpf were extracted by Folch method and analyzed by high-performance thin layer chromatography (HPTLC) as wide-range preliminary screening. Selected conditions (0 and 6 mg/L of BPA) were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC-TOFMS). BPA exposed ZE exhibited increased amounts of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), regarding the control group. Analysis of time- and BPA exposure-related patterns of specific lipid species showed a clear influence of unsaturation degree (mostly in DG and PC) and/or fatty acid chain length (mostly in TG and PC derivatives) on their response to the presence of BPA. A decreased yolk-sac and energy consumption in exposed individuals appeared as the main reason for the observed BPA-driven effects. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests a disruption of different signalling pathways by BPA that starts at very low BPAconcentrations, whose effects propagate across different organization levels, and that cannot be only explained by the relatively weak estrogenic effect of BPA. © 2019 Elsevier Ltd | Versión del editor: | https://doi.org/10.1016/j.chemosphere.2019.125704 | URI: | http://hdl.handle.net/10261/197531 | DOI: | 10.1016/j.chemosphere.2019.125704 |
Aparece en las colecciones: | (IDAEA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage.pdf | Artículo principal | 3,01 MB | Adobe PDF | Visualizar/Abrir |
1-s2.0-S0045653519329455-mmc1.xlsx | Material suplementario | 120,64 kB | Microsoft Excel XML | Visualizar/Abrir |
1-s2.0-S0045653519329455-mmc2.docx | Material suplementario | 627,84 kB | Microsoft Word XML | Visualizar/Abrir |
1-s2.0-S0045653519329455-mmc3.pdf | Material suplementario | 1,39 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
30
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
24
checked on 24-feb-2024
Page view(s)
335
checked on 26-abr-2024
Download(s)
350
checked on 26-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.