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| Título: | Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs |
Autor: | Gandasegui, Javier CSIC ORCID ; Grau-Pujol, B.; Cambra Pelletjá, María CSIC ORCID; Escola, V.; Demontis, M. A.; Cossa, A.; Jamine, J.C.; Balaña-Fouce, Rafael; Van Lieshout, L.; Muñoz, J.; Martínez Valladares, María CSIC ORCID CVN | Palabras clave: | Soil-transmitted helminths Benzimidazoles Anthelmintic resistance Pyrosequencing |
Fecha de publicación: | 2021 | Editor: | Asís Biomedia | Citación: | Parasites & vectors, 14(1): 490 (2021) | Resumen: | There is an urgent need for an extensive evaluation of benzimidazole efcacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the stool sample processing methodology and resistance allele frequency assessment in Trichuris trichiura and Necator americanus anthelmintic-related SNPs by pyrosequenc‑ ing, and standardized it for large-scale benzimidazole efcacy screening use. | Descripción: | 12 páginas, 3 tablas, 3 figuras. | Versión del editor: | http://dx.doi.org/10.1186/s13071-021-04941-w | URI: | http://hdl.handle.net/10261/251424 | E-ISSN: | 1756-3305 |
| Aparece en las colecciones: | (IGM) Artículos |
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| Martinez_Valladares_Improving_Parasites & vectors_2021_s13071-021-04941-w.pdf | Artículo prncipal | 1,11 MB | Adobe PDF |  Visualizar/Abrir |
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