Infection of mitogen‐stimulated trout leucocytes with salmonid viruses

Abstract

Abstract. The effects of infection with salmonid viruses on mitogen‐stimulated cells and colonies from rainbow trout, Oncorhynchus imkiss (Richardson), kidney cell cultures have been studied using culture in clots of fibrin. Phytohaemaglutinin, concanavalin A and lypo‐polysaccharides from Escherichia coli have been used as mitogens. The viral haemorrhagic septicaemia (VHS) virus destroyed any mitogen‐induced trout kidney colonies or cells, but the infectious pancreatic necrosis (IPN) virus caused no cell or colony death, and even increased their count in the cultures. The results suggested that the target for IPN virus replication is not any trout leucocyte but that among the possible targets of VHS virus are the different types of leucocytes found in the trout kidney and in their in vitro mitogen‐stimulated colonies. Copyright © 1991, Wiley Blackwell. All rights reserved