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Título: | Bacteria in bovine semen can increase sperm DNA fragmentation rates A kinetic experimental approach |
Autor: | González-Marín, C.; Roy, R.; López-Fernández, C.; Diez, Begoña; Carabaño Luengo, María Jesús; Fernández, José L.; Kjelland, M. E.; Moreno, Juan Fernando; Gosálvez, J. | Palabras clave: | Sperm DNA fragmentation Bacterial infection Artificial reproduction techniques Male factor |
Fecha de publicación: | 2011 | Editor: | Elsevier | Citación: | Animal Reproduction Science 123(3-4): 139-148 (2011) | Resumen: | Cryopreserved straws of semen (n=228) from Holstein bulls (n=47) were examined for bacterial presence and sperm DNA fragmentation (SDF) dynamics. Commercial semen doses (representing six ejaculates per individual) were randomly selected from a bull stud in Spain. The dynamics of SDF were assessed after thawing (T0) and at 4, 24, 48, 72 and 96h of incubation at 37°C, using the commercial variant of the sperm chromatin dispersion test for Bovine (Halomax®). One group of bulls showed a bacterial presence in semen samples between 0 and 96h of incubation (n=23, group A) while the other did not (n=24, group B). Immediate post-thaw differences in SDF were not observed when both groups were compared. However, the rate of increase in SDF (rSDF) over time, considered as an estimate of the kinetic behaviour of sperm DNA survival, was significantly higher (P<0.05) in semen samples from group A (0.7% per hour) versus group B (0.05% per hour). Polymerase Chain Reaction (PCR) assay was used for DNA amplification using primers designed for specific regions of the bacterial gene that codifies for 16S rRNA. Different species within the phyla Bacteroidetes, Firmicutes, Proteobacteria, Cyanobacteria, Fusobacteria and Actinobacteria were identified. The results show that (1) SDF at baseline (T0) may not be affected by the presence of bacteria but the rSDF can increase due to bacterial growth during incubation, (2) the increase in the rSDF is characteristic of some bulls but not for others, and (3) certain bacterial strains are repeatedly found in separate ejaculates from the same bull. © 2010 Elsevier B.V. | URI: | http://hdl.handle.net/10261/291428 | DOI: | 10.1016/j.anireprosci.2010.11.014 | ISSN: | 0378-4320 |
Aparece en las colecciones: | (INIA) Artículos |
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