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Título: | Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts |
Autor: | Bermejo Álvarez, Pablo; Rizos, Dimitrios; Rath, D.; Lonergan, P.; Gutiérrez-Adán, Alfonso CSIC ORCID | Palabras clave: | Gender Preimplantation Microarray Imprinting X-inactivation |
Fecha de publicación: | 2010 | Editor: | National Academy of Sciences (U.S.) | Citación: | PNAS 107(8): 3394-3399 (2010) | Resumen: | Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the mRNA level, we compared gene-expression patterns between male and female blastocysts by DNA microarray comparison of nine groups of 60 bovine in vitro-produced blastocysts of each sex. Almost one-third of the transcripts detected showed sexual dimorphism (2,921 transcripts; false-discovery rate, P < 0.05), suggesting that in the absence of hormonal influences, the sex Chrs impose an extensive transcriptional regulation upon autosomal genes. Six geneswere analyzed by qPCR in in vivo-derived embryos, which displayed similar sexual dimorphism. Ontology analysis suggested a higher global transcriptional level in females andamore active proteinmetabolisminmales. Agenehomologto an X-linkedgeneinvolved in network interactions during spliceosome assembly was found in the Y-Chr. Most of the X-linked-expressed transcripts (88.5%)were up-regulated in females, but most of them (70%) exhibited fold-changes lower than 1.6, suggesting that X-Chr inactivation is partially achieved at the blastocyst stage. Almost half of the transcripts up-regulated in female embryos exhibiting more than 1.6-fold change were present in the X-Chr and eight of themwere selected to determinea putativepaternal imprinting by gene expression comparison with parthenogenetic embryos. Five (BEX, CAPN6, BEX2, SRPX2, and UBE2A) exhibited a higher expression in females than in parthenotes, suggesting that they are predominantly expressed by the paternal inherited X-Chr and that imprinting may increase the transcriptional skew caused by double X-Chr dosage. | URI: | http://hdl.handle.net/10261/292193 | DOI: | 10.1073/pnas.0913843107 | ISSN: | 0027-8424 | E-ISSN: | 1091-6490 |
Aparece en las colecciones: | (INIA) Artículos |
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