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Título: | A role for the gene regulatory module microRNA172/TARGET OF EARLY ACTIVATION TAGGED 1/FLOWERING LOCUS T (miRNA172/TOE1/FT) in the feeding sites induced by Meloidogyne javanica in Arabidopsis thaliana |
Autor: | Díaz-Manzano, Fernando Evaristo; Cabrera, Javier; Ripoll, Juan José; del Olmo, I.; Andrés, María Fé; Silva, Ana Cláudia; Barcala, M.; Sánchez, María; Ruiz-Ferrer, Virginia CSIC ORCID; de Almeida-Engler, J.; Yanofsky, M. F.; Piñeiro Galvin, Manuel Ángel; Jarillo, José Antonio; Fenoll, Carmen; Escobar, Carolina | Palabras clave: | AP2 Arabidopsis Flowering Giant cells Meloidogyne spp miRNA172 Repression |
Fecha de publicación: | 2018 | Editor: | John Wiley & Sons | Citación: | New Phytologist 217(2): 813-827 (2018) | Resumen: | Root knot nematodes (RKNs) penetrate into the root vascular cylinder, triggering morphogenetic changes to induce galls, de novo formed 'pseudo-organs' containing several giant cells (GCs). Distinctive gene repression events observed in early gall/GCs development are thought to be mediated by post-transcriptional silencing via microRNAs (miRNAs), a process that is far from being fully characterized. Arabidopsis thaliana backgrounds with altered activities based on target 35S::MIMICRY172 (MIM172), 35S::TARGET OF EARLY ACTIVATION TAGGED 1 (TOE1)-miR172-resistant (35S::TOE1R ) and mutant (flowering locus T-10 (ft-10)) lines were used for functional analysis of nematode infective and reproductive parameters. The GUS-reporter lines, MIR172A-E::GUS, treated with auxin (IAA) and an auxin-inhibitor (a-(phenyl ethyl-2-one)-indole-3-acetic acid (PEO-IAA)), together with the MIR172C AuxRE::GUS line with two mutated auxin responsive elements (AuxREs), were assayed for nematode-dependent gene expression. Arabidopsis thaliana backgrounds with altered expression of miRNA172, TOE1 or FT showed lower susceptibility to the RKNs and smaller galls and GCs. MIR172C-D::GUS showed restricted promoter activity in galls/GCs that was regulated by auxins through auxin-responsive factors. IAA induced their activity in galls while PEO-IAA treatment and mutations in AuxRe motifs abolished it. The results showed that the regulatory module miRNA172/TOE1/FT plays an important role in correct GCs and gall development, where miRNA172 is modulated by auxins. | URI: | http://hdl.handle.net/10261/292947 | DOI: | 10.1111/nph.14839 | ISSN: | 0028-646X | E-ISSN: | 1469-8137 |
Aparece en las colecciones: | (INIA) Artículos |
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