Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/293612
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Genetic stability analysis of chrysanthemum (Chrysanthemum x morifolium Ramat) after different stages of an encapsulation-dehydration cryopreservation protocol |
Autor: | Martín, Carmen CSIC ORCID; Cervera, María Teresa; González-Benito, M. E. | Palabras clave: | AFLP Chrysanthemum Cryopreservation Genetic stability RAPD |
Fecha de publicación: | 2011 | Editor: | Elsevier | Citación: | Journal of Plant Physiology 168(2): 158-166 (2011) | Resumen: | Genetic stability in chrysanthemum (cultivar 'Pasodoble') apices was studied at each step of an encapsulation-dehydration cryopreservation protocol control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and cryopreservation (Cr). Two different markers were employed RAPDs and AFLPs. Throughout the process, the origin of the apices (in vitro shoot from which they were excised) was recorded. Eight complete lines (from which DNA could be amplified after all the steps considered) were studied. Two out of twelve arbitrary primers showed polymorphisms. Three RAPD markers were replaced by three new ones in the Cr sample in one line. Using a different primer, a 700 bp fragment was absent from all samples from the 0.3. M sucrose-culture step ('P') onwards, in all the lines studied. The sequences of these fragments were studied to find similarities with known sequences. Polymorphic AFLP fragments were also observed, and most of the differences appeared from step 'P' onwards, pointing out the possible effect of this process (preculture on 0.3. M sucrose) in the DNA variation. These results show that genetic variation can appear throughout the cryopreservation process, and the low temperature itself is not the only stress risk of the technique. Therefore, genetic stability of the regenerants obtained after cryopreservation should be monitored. © 2010 Elsevier GmbH. | URI: | http://hdl.handle.net/10261/293612 | DOI: | 10.1016/j.jplph.2010.06.025 | ISSN: | 0176-1617 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
45
checked on 07-may-2024
WEB OF SCIENCETM
Citations
44
checked on 15-feb-2024
Page view(s)
16
checked on 15-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.