Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/294775
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Report on the analyses of mAb reactive with porcine CD8 for the Second International Swine CD Workshop

AutorZuckermann, F.; Pescovitz, M. D.; Aasted, B.; Domínguez, Javier; Trebichavsky, I.; Novikov, B.; Valpotic, I.; Nielsen, Jens; Arn, S.; Sachs, David H.; Lunney, J. K.; Boyd, P. C.; Walker, J. CSIC; Lee, Rogan; Davis, William; Barbosa, I. R.; Saalmüller, A.
Palabras clavePorcine CD8
Peripheral blood lymphocytes
Two-color cytofluorometry
Fecha de publicación1998
EditorElsevier
CitaciónVeterinary Immunology and Immunopathology 60(3-4): 291-303 (1998)
ResumenBased on an analysis of their reactivity with porcine peripheral blood lymphocytes (PBL), only three of the 57 mAbs assigned to the T cell/activation marker group were grouped into cluster T9 along with the two wCD8 workshop standard mAbs 76-2-11 (CD8a) and 11/295/33 (CD8b). Their placement was verified through the use of two-color cytofluorometry which established that all three mAbs (STH101, 090; UCP1H12-2, 139, and PG164A, 051) bind exclusively to CD8+ cells. Moreover, like the CD8 standard mAbs, these three mAbs reacted with two proteins with a MW of 3-3 and 35 kDa from lymphocyte lysates and were, thus, given the wCD8 designation. Because the mAb STH101 inhibited the binding of mAb 76-2-11 but not of 11/295/33, it was given the wCD8a designation. The reactivity of the other two new mAbs in the T9 cluster with the various subsets of CD8+ lymphocytes were distinct from that of the other members in this cluster including the standards. Although the characteristic porcine CD8 staining pattern consisting of CD8(low) and CD8(high) cells was obtained with the mAb UCP1H12-2, a wider gap between the fluorescence intensity of the CD8(low) and CD8(high) lymphocytes was observed. In contrast, the mAb PG164A, not only exclusively reacted with CD4-/CD8(high) lymphocytes, but it also failed to recognize CD4/CD8 double positive lymphocytes. It was concluded that this mAb is specific for a previously unrecognized CD8 epitope, and was, thus, given the wCD8c designation. A very similar reactivity pattern to that of PG164A was observed for two other mAbs (STH106, 094; and SwNL554.1, 009). Although these two mAbs were not originally positioned in the T cell subgroup because of their reactivity and their ability to inhibit the binding of PG164A, they were given the wCD8c designation. Overall, five new wCD8 mAbs were identified. Although the molecular basis for the differences in PBL recognition by these mAbs is not yet understood, they will be important in defining the role of CD8+ lymphocyte subsets in health and disease.
URIhttp://hdl.handle.net/10261/294775
DOI10.1016/S0165-2427(97)00106-2
ISSN0165-2427
Aparece en las colecciones: (INIA) Artículos

Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

39
checked on 25-may-2024

WEB OF SCIENCETM
Citations

37
checked on 29-feb-2024

Page view(s)

36
checked on 27-may-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.