LPS-induced cochlear inflammation and SPT-2101 treatment

Murillo-Cuesta, Silvia; Lara, Ester; Bermúdez-Muñoz, Jose Mª; Torres-Campos, Elena; Rodriguez-de la Rosa, Lourdes; Varela-Nieto, Isabel

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Título:	LPS-induced cochlear inflammation and SPT-2101 treatment
Autor:	Murillo-Cuesta, Silvia CSIC ORCID; Lara, Ester; Bermúdez-Muñoz, Jose Mª CSIC ORCID; Torres-Campos, Elena; Rodriguez-de la Rosa, Lourdes CSIC ORCID; Varela-Nieto, Isabel CSIC ORCID
Fecha de publicación:	21-mar-2023
Editor:	DIGITAL.CSIC
Citación:	Murillo-Cuesta, Silvia; Lara, Ester; Bermúdez-Muñoz, Jose Mª; Torres-Campos, Elena; Rodriguez-de la Rosa, Lourdes; Varela-Nieto, Isabel; 2023; LPS-induced cochlear inflammation and SPT-2101 treatment [Dataset]; DIGITAL.CSIC; https://doi.org/10.20350/digitalCSIC/15167
Descripción:	[Methods] ABR DATA: obtained by ABR recording in a Tucker David Technology RZ6 Auditory Workstation using BioSigRZ software EVANS BLUE DATA: obtained by fluorimetry in a Glomax™ Luminometer and Microplate Reader (Promega) (620/680 nm excitation/emission wavelength). GENE EXPRESSION DATA: obtained by Real-Time PCR and analyzed by QuantStudio™ Real-Time PCR software 1.3. PCR array obtained using the PCR array RT² Profiler™ Rat Innate & Adaptive Immune Responses (Qiagen, #PARN-052Z) and analyzed with Geneglobe (Qiagen). MRI DATA: images obtained with a horizontal 7.0-Tesla Bruker BioSpec® system (Bruker Medical GmbH) running ParaVision 6.0.1 on a Linux environment. Data analysis was performed with Fiji software. OPTICAL IMAGING DATA: images obtained with a Zeiss AxioPhot microscope (Carl Zeiss). Data analysis was performed with Fiji software. FLUORESCENCE DATA: images obtained with an epifluorescence (Nikon 90i) and confocal laser-scanning (Zeiss LSM710) microscopes. Data analysis was performed with Fiji software. WB DATA: Immunoreactive bands revealed using the Clarity™ Western ECL Substrate (Bio-Rad), and images were captured with the ImageQuant LAS4000 mini digital camera using ImageQuant TL software 8.1 (GE Healthcare).
URI:	http://hdl.handle.net/10261/303663
DOI:	https://doi.org/10.20350/digitalCSIC/15167
Referencias:	Murillo-Cuesta, Silvia; Lara, Ester; Bermúdez-Muñoz, Jose Mª; Torres-Campos, Elena; Rodriguez-de la Rosa, Lourdes; López-Larrubia, Pilar; Erickson, Signe R.; Varela-Nieto, Isabel. Protection of lipopolysaccharide-induced otic injury by a single dose administration of a novel dexamethasone formulation. https://doi.org/10.1186/s41231-023-00156-6 . http://hdl.handle.net/10261/337084
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