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Título: | Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
Autor: | Hoste, Alexis C.R.; Venteo, Ángel; Fresco-Taboada, Alba; Tapia, Istar; Monedero, Alejandro; López, Lissette; Jebbink, Maarten F.; Pérez-Ramírez, E. CSIC ORCID; Jiménez-Clavero, Miguel Ángel CSIC ORCID ; Almonacid, Mercedes; Muñóz, Patricia E.; Guinea, Jesus; Vela, Carmen; van der Hoek, Lia; Rueda, Paloma; Sastre, Patricia | Palabras clave: | COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology |
Fecha de publicación: | dic-2020 | Editor: | Elsevier | Citación: | Diagnostic Microbiology and Infectious Disease 98(4): e115167 (2020) | Resumen: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than 8 million people worldwide, becoming a pandemic. Detecting antibodies against SARS-CoV-2 is of utmost importance and a good indicator of exposure and circulation of the virus within the general population. Two serological tools based on a double recognition assay [enzyme-linked immunosorbent assay (DR-ELISA) and lateral flow assay (DR-LFA)] to detect total antibodies to SARS-CoV-2 have been developed based on the recombinant nucleocapsid protein. A total of 1065 serum samples, including positive for COVID-19 and negative samples from healthy donors or infected with other respiratory pathogens, were analyzed. The results showed values of sensitivity between 91.2% and 100%, and specificity of 100% and 98.2% for DR-LFA and DR-ELISA, respectively. No cross-reactivity against seasonal coronavirus (HCoV-NL63, HCoV-229E, HCoV-HKU1, HCoV-OC43) was found. These results demonstrate the importance of serology as a complementary tool to polymerase chain reaction for follow-up of recovered patients and identification of asymptomatic individuals. | Descripción: | 5 Pág. | Versión del editor: | https://doi.org/10.1016/j.diagmicrobio.2020.115167 | URI: | http://hdl.handle.net/10261/337345 | DOI: | 10.1016/j.diagmicrobio.2020.115167 | ISSN: | 0732-8893 |
Aparece en las colecciones: | (INIA) Artículos (PTI Salud Global) Colección Especial COVID-19 |
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