Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/95584
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA |
Autor: | Solano-Collado, Virtu CSIC ORCID; Lurz, Rudi; Espinosa, Manuel CSIC ORCID ; Bravo, Alicia CSIC ORCID | Palabras clave: | Bacterial proteins DNA footprinting Operon Promoter regions Genetic Transcription factors |
Fecha de publicación: | 2013 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 41: 6975- 6991 (2013) | Resumen: | The MgaSpn transcriptional regulator contributes to the virulence of Streptococcus pneumoniae. It is thought to be a member of the Mga/AtxA family of global regulators. MgaSpn was shown to activate in vivo the P1623B promoter, which is divergent from the promoter (Pmga) of its own gene. This activation required a 70-bp region (PB activation region) located between both promoters. In this work, we purified an untagged form of the MgaSpn protein, which formed dimers in solution. By gel retardation and footprinting assays, we analysed the binding of MgaSpn to linear double-stranded DNAs. MgaSpn interacted with the PB activation region when it was placed at internal position on the DNA. However, when it was positioned at one DNA end, MgaSpn recognized preferentially the Pmga promoter placed at internal position. In both cases, and on binding to the primary site, MgaSpn spread along the adjacent DNA regions generating multimeric protein-DNA complexes. When both MgaSpn-binding sites were located at internal positions on longer DNAs, electron microscopy experiments demonstrated that the PB activation region was the preferred target. DNA molecules totally or partially covered by MgaSpn were also visualized. Our results suggest that MgaSpn might recognize particular DNA conformations to achieve DNA-binding specificity | Descripción: | Este artículo pertenece a la Tesis presentada por Virtudes Solano Collado con título: "Caracterización molecular del regulador transcripcional MgaSpn de Streptococcus pneumoniae"(https://digital.csic.es/handle/10261/98162) | Versión del editor: | http://dx.doi.org/10.1093/nar/gkt445 | URI: | http://hdl.handle.net/10261/95584 | DOI: | 10.1093/nar/gkt445 | ISSN: | 0305-1048 | E-ISSN: | 1362-4962 |
Aparece en las colecciones: | (CAB) Artículos (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Nucl. Acids Res.-2013-Solano-Collado-6975-91.pdf | 10,76 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
15
checked on 26-abr-2024
SCOPUSTM
Citations
17
checked on 24-abr-2024
WEB OF SCIENCETM
Citations
16
checked on 28-feb-2024
Page view(s)
227
checked on 24-abr-2024
Download(s)
204
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.