Compartir
Título
β-(1,4)-Galactan remodelling in Arabidopsis cell walls affects the xyloglucan structure during elongation
Autor(es)
Palabras clave
β-Galactosidases
Growth
Pectin
Fecha de publicación
2019
Resumen
β-(1,4)-Galactan is one of the main side chains of rhamnogalacturonan I. Although the specific function of this polymer has
not been completely established, it has been related to different developmental processes. To study β-(1,4)-galactan function,
we have generated transgenic Arabidopsis plants overproducing chickpea βI-Gal β-galactosidase under the 35S CaMV
promoter (35S::βI-Gal) to reduce galactan side chains in muro. Likewise, an Arabidopsis double loss-of-function mutant for
BGAL1 and BGAL3 Arabidopsis β-galactosidases (bgal1/bgal3) has been obtained to increase galactan levels. The characterization
of these plants has confirmed the role of β-(1,4)-galactan in cell growth, and demonstrated that the turnover of this
pectic side chain occurs during cell elongation, at least in Arabidopsis etiolated hypocotyls and floral stem internodes. The
results indicate that BGAL1 and BGAL3 β-galactosidases act in a coordinate way during cell elongation. In addition, this
work indicates that galactan plays a role in the maintenance of the cell wall architecture during this process. Our results point
to an involvement of the β-(1,4)-galactan in the xyloglucan structure and the interaction between cellulose and xyloglucan
URI
ISSN
0032-0935
DOI
10.1007/s00425-018-3008-5
Versión del editor
Aparece en las colecciones
- GIPACYCV. Artículos [59]
Ficheros en el ítem
Nombre:
Galactan remodelling and xyloglucan_Planta 2019.pdfEmbargado hasta: 2099-09-09
Tamaño:
1.544Mb
Formato:
Adobe PDF
Descripción:
Artículo de investigación