Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10400.1/14946
Título: | Extracellular multi-unit recording from the olfactory nerve of teleosts |
Autor: | Hubbard, Peter Velez, Zélia |
Palavras-chave: | Animals Carbon dioxide Electrophysiological phenomena Extracellular space Fishes Glutamine Hydrogen-ion concentration Leucine Olfactory nerve Seawater Smell Software |
Data: | 2020 |
Editora: | MyJove Corporation |
Resumo: | Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording from the olfactory nerve is the method of choice. Although invasive, it is sensitive, robust, reproducible and independent of external salinity (unlike the electro-olfactogram [EOG], for example). Furthermore, it records a primary sensory input into the CNS, prior to any central processing. We show that this method can show a reduction in olfactory sensitivity that is both temporary and odorant-dependent, using a range of amino acids to construct concentration-response curves and calculate the thresholds of detection. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.1/14946 |
DOI: | 10.3791/60962 |
ISSN: | 1940-087X |
Aparece nas colecções: | CCM2-Artigos (em revistas ou actas indexadas) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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jove-protocol-60962-extracellular-multi-unit-recording-from-olfactory-nerve.pdf | 638,31 kB | Adobe PDF | Ver/Abrir |
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