Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression

Moutou, K. A.; Socorro, Sílvia; Power, Deborah; Mamuris, Z.; Canario, Adelino V. M.

http://hdl.handle.net/10400.1/3951

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Authors

Canario, Adelino V. M.

Abstract(s)

In the present study, the Sparus aurata -skeletal actin was cloned from a mixed larvae complementary DNA library. The clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. The deduced amino acid sequence of sea bream -actin is identical to Fugu -actin-1. The expression of -actin was initiated at the onset of segmentation. In adult fish, -actin is expressed predominantly in white and red muscle.

Keywords

Teleost Skeletal muscle Actin cDNA Gilthead sea bream ŽSparus aurata

URI

http://hdl.handle.net/10400.1/3951

Citation

Moutou, K. A.; Socorro, S.; Power, D. M.; Mamuris, Z.; Canario, A. V. M. Molecular cloning and sequence of gilthead sea bream (Sparus aurata) alpha-skeletal actin: tissue and developmental expression, Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 130, 1, 13-21, 2001.