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http://hdl.handle.net/10400.18/3433
Título: | Helicobacter pullorum Isolated from Fresh Chicken Meat: Antibiotic Resistance and Genomic Traits of an Emerging Foodborne Pathogen |
Autor: | Borges, Vítor Santos, Andrea Correia, Cristina Belo Saraiva, Margarida Ménard, Armelle Vieira, Luís Sampaio, Daniel A. Pinheiro, Miguel Gomes, João Paulo Oleastro, Mónica |
Palavras-chave: | Helicobacter pullorum Emergent Foodborne Pathogen Virulence Antibiotic Resistance Chicken Meat Whole Genome Sequencing Infecções Gastrointestinais |
Data: | Dez-2015 |
Editora: | American Society for Microbiology |
Citação: | Appl Environ Microbiol. 2015 Dec;81(23):8155-63. doi: 10.1128/AEM.02394-15. Epub 2015 Sep 18 |
Resumo: | Meat and meat products are important sources of human intestinal infections. We report the isolation of Helicobacter pullorum strains from chicken meat. Bacteria were isolated from 4 of the 17 analyzed fresh chicken meat samples, using a membrane filter method. MIC determination revealed that the four strains showed acquired resistance to ciprofloxacin; one was also resistant to erythromycin, and another one was resistant to tetracycline. Whole-genome sequencing of the four strains and comparative genomics revealed important genetic traits within the H. pullorum species, such as 18 highly polymorphic genes (including a putative new cytotoxin gene), plasmids, prophages, and a complete type VI secretion system (T6SS). The T6SS was found in three out of the four isolates, suggesting that it may play a role in H. pullorum pathogenicity and diversity. This study suggests that the emerging pathogen H. pullorum can be transmitted to humans by chicken meat consumption/contact and constitutes an important contribution toward a better knowledge of the genetic diversity within the H. pullorum species. In addition, some genetic traits found in the four strains provide relevant clues to how this species may promote adaptation and virulence. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.18/3433 |
DOI: | 10.1128/AEM.02394-15 |
ISSN: | 0099-2240 |
Versão do Editor: | http://aem.asm.org/content/81/23/8155.abstract |
Aparece nas colecções: | DDI - Artigos em revistas internacionais DAN - Artigos em revistas internacionais DGH - Artigos em revistas internacionais |
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borges2015.pdf | 1,03 MB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
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