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http://hdl.handle.net/10400.18/6342
Título: | Measurement of adsorption constants of laccase on gold nanoparticles to evaluate the enhancement in enzyme activity of adsorbed laccase |
Autor: | Peixoto de Almeida, Miguel Quaresma, Pedro Sousa, Susana Couto, Cláudia Gomes, Inês Krippahl, Ludwig Franco, Ricardo Pereira, Eulália |
Palavras-chave: | Adsorption Gold Hydrazones Hydrogen-Ion Concentration Kinetics Laccase Metal Nanoparticles Toxicodendron Genotoxicidade Ambiental |
Data: | 20-Jun-2018 |
Editora: | Royal Society of Chemistry |
Citação: | Phys Chem Chem Phys. 2018 Jun 20;20(24):16761-16769. doi: 10.1039/c8cp03116a |
Resumo: | Adsorption of enzymes to nanoparticles and the mechanisms responsible for enzyme activity modulation of adsorbed enzymes are not well understood. In this work, gold nanoparticles were used for electrostatic adsorption of a plant-derived laccase. Adsorption constants were determined by four independent techniques: dynamic light scattering, electrophoretic light scattering, agarose gel electrophoresis and fluorescence quenching. Stable bionanoconjugates were formed with log K in the range 6.8-8.9. An increase in enzyme activity was detected, in particular at acidic and close to neutral pH values, a feature that expands the useful pH range of the enzyme. A model for the adsorption was developed, based on geometrical considerations and volume increase data from dynamic light scattering. This indicates that enzymes adsorbed to gold nanoparticles are ca. 9 times more active than the free enzyme. |
Descrição: | Susana Sousa, Departamento de Doenças Infeciosas, Instituto Nacional de Saúde Doutor Ricardo Jorge, Porto, Portugal |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.18/6342 |
DOI: | 10.1039/c8cp03116a |
ISSN: | 1463-9076 |
Versão do Editor: | https://pubs.rsc.org/en/Content/ArticleLanding/2018/CP/C8CP03116A#!divAbstract |
Aparece nas colecções: | DDI - Artigos em revistas internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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32_measurement.pdf | 1,03 MB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! | |
32_suppl.pdf | 806,44 kB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
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