Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10400.22/23880
Título: | Nanostructured label–free electrochemical immunosensor for detection of a Parkinson's disease biomarker |
Autor: | Carneiro, Pedro Loureiro, Joana A. Delerue-Matos, Cristina Morais, Simone Pereira, Maria do Carmo |
Palavras-chave: | α-synuclein Gold nanoparticles Carbon nanotubes Monoclonal antibody |
Data: | 2023 |
Editora: | Elsevier |
Resumo: | Aggregation of α-synuclein has been recognized as a critical event in the pathogenesis of Parkinson's disease whose prevalence is increasing with great socio-economic challenges for future generations. Here, we developed a sensitive and specific electrochemical immunosensor for the detection and quantification of this biomarker, based on the voltammetric study of a redox indicator signal, which decreases upon the analyte recognition by the antibody due to the electronic resistance increase. The proposed immunosensor is based on a screen-printed carbon electrode modified in a layer-by-layer approach, which through extensive characterization led to the successful nanostructuration of the transducer, through the drop-cast of 3.0 μL of a 0.1 mg mL−1 single-walled carbon nanotubes suspension followed by electrodeposition of gold nanoparticles in a 3 mM HAuCl4 solution under a −0.2 V potential for 150 s. Monoclonal antibodies were immobilized on the gold nanoparticles surface through chemical modification at an optimal concentration of 200 μg mL−1. Using the proposed immunosensor, α-synuclein was detected in the range of 0.01–10 ng mL−1 with a 4.1 and 12.6 pg mL−1 limits of detection and quantification, respectively. Recovery values of 96.7, 106.2 and 102.9% were attained for the tested concentrations spiked in fetal bovine serum while also presenting excellent specificity and stability throughout one month. The nanostructured immunosensor provided a great interface for electronic transduction and biological recognition events, which enabled fast, sensitive and specific detection of α-synuclein while being based on a simple and inexpensive technology requiring small sample volumes, crucial characteristics for application in point-of-care testing. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.22/23880 |
DOI: | 10.1016/j.talanta.2022.123838 |
ISSN: | 00399140 |
Versão do Editor: | https://www.sciencedirect.com/science/article/pii/S0039914022006348 |
Aparece nas colecções: | ISEP – GRAQ – Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ART_GRAQ_CMM_2023.pdf | 2,64 MB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.