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http://hdl.handle.net/10400.22/7405
Título: | ABCC Subfamily Vacuolar Transporters are Involved in Pb (Lead) Detoxification in Saccharomyces cerevisiae |
Autor: | Sousa, Cátia A. Hanselaer, Simon Soares, Eduardo V. |
Palavras-chave: | Glutathione transferases (GSTs) Heavy metal Lead (Pb) Vacuolar ABC transporters Yeast |
Data: | 2015 |
Editora: | Springer |
Relatório da Série N.º: | Applied Biochemistry and Biotechnology;Vol. 175, Issue 1 |
Resumo: | The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased susceptibility to high Pb concentrations (500-1000 μmol L(-1)). These results suggest that the formation of glutathione-Pb conjugate (GS-Pb), dependent of GSTs, is important in Pb detoxification. The involvement of ATP-binding cassette (ABC) vacuolar transporters, belonging to class C subfamily (ABCC) in vacuolar compartmentalization of Pb, was evaluated. For this purpose, mutant strains disrupted in YCF1, VMR1, YBT1 or BPT 1 genes were used. All mutants tested, without vacuolar ABCC transporters, presented an increased sensitivity to 500-1000 μmol L(-1) Pb comparative to wild-type strain. Taken together, the obtained results suggest that Pb detoxification, by vacuolar compartmentalization, can occur as a result of the concerted action of GSTs and vacuolar ABCC transporters. Pb is conjugated with glutathione, catalysed by glutathione transferases and followed to the transport of GS-Pb conjugate to the vacuole by ABCC transporters. |
Peer review: | yes |
URI: | http://hdl.handle.net/10400.22/7405 |
DOI: | 10.1007/s12010-014-1252-0 |
Versão do Editor: | http://link.springer.com/article/10.1007%2Fs12010-014-1252-0 |
Aparece nas colecções: | ISEP – CIETI – Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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ABCC Subfamily Vacuolar Transporters are Involved in Pb.pdf | 622,72 kB | Adobe PDF | Ver/Abrir |
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