Spectral and thermal data as a proxy for leaf protective energy dissipation under kaolin application in grapevine cultivars

Abstract

The dynamic effects of kaolin clay particle film application on the temperature and spectral reflectance of leaves of two autochthonous cultivars (Touriga Nacional (TN, n=32) and Touriga Franca (TF, n=24)) were studied in the Douro wine region. The study was implemented in 2017, in conditions prone to multiple environmental stresses that include excessive light and temperature as well as water shortage. Light reflectance from kaolin-sprayed leaves was higher than the control (leaves without kaolin) on all dates. Kaolin’s protective effect over leaves’ temperatures was low on the 20 days after application and ceased about 60 days after its application. Differences between leaves with and without kaolin were explained by the normalized maximum leaf temperature (T_max_f_N), reflectance at 400 nm, 532 nm, and 737 nm, as assessed through TN data. The wavelengths of 532 nm and 737 nm are associated with plant physiological processes, which support the selection of these variables for assessing kaolin’s effects on leaves. The application of principal component analysis to the TF data, based on these four variables (T_max_f_N and reflectances: 400, 532, 737 nm) selected for TN, explained 83.56% of data variability (considering two principal components), obtaining a clear differentiation between leaves with and without kaolin. The T_max_f_N and the reflectance at 532 nm were the variables with a greater contribution for explaining data variability. The results improve the understanding of the vines’ response to kaolin throughout the grapevine cycle and support decisions about the re-application timing