Efeito da criopreservação no sémen de caprinos da raça serpentina através de avaliação microscópica e do computer-assisted sperm analysis (CASA)
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2019
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Atualmente, a tecnologia do CASA dispõe de diversas vantagens, contudo ainda não existe uma padronização dos parâmetros específicos para cada espécie animal, devido à escassa ou incompleta bibliografia.
Pretendeu-se com este estudo, a avaliação da viabilidade do sémen criopreservado de 9 bodes Serpentinos através da análise do Computer Assisted Sperm Analysis (CASA). No que diz respeito à motilidade, Total e Progressiva, observou-se 49.99% ± 17.62% e 13.93% ± 9.04%, respetivamente. Os valores médios das velocidades: VCL, VSL e VAP foram de 76.29%, 37.04%, e 50.03%. A observação da morfologia revelou 75% ± 10.48% de formas normais à descongelação, sendo os defeitos de cauda a anomalia mais comum encontrada (14.61% ± 5.12%), observando-se uma relação entre esta anomalia e a motilidade total (r = -0.521; P-value = 0.001). A comparação entre os resultados da motilidade obtidos através do método subjetivo (40.42% ± 15.04%) e da análise pelo sistema CASA (49.99% ± 17.62%), revelou uma correlação altamente significativa entre ambas (r = 0,802; P-value <0,0001).
Os resultados, apesar de não conclusivos na totalidade, devido não só às diversas dúvidas existentes sobre o CASA, mas também pela reduzida amostra populacional, revelam que existe uma grande discrepância entre o valor da motilidade subjetiva e o valor dos espermatozóides móveis progressivos e evidenciam que a avaliação feita por um Técnico se aproxima muito mais do valor da motilidade total, sendo essencial uma maior pesquisa e utilização dos sistemas CASA no futuro da criopreservação.
Currently, CASA technology has several advantages, yet there is no standardization of specific parameters for each animal species, due to the scarce or incomplete bibliography. The aim of this study was to evaluate the viability of cryopreserved semen from 9 Serpentine bucks through the Computer Assisted Sperm Analysis (CASA). Regarding motility, Total and Progressive were 49.99% ± 17.62% and 13.93% ± 9.04%, respectively. The average velocity values: VCL, VSL and VAP were 76.29%, 37.04%, and 50.03%. Observation of morphology revealed 75% ± 10.48% of normal defrost forms, with tail defects being the most common anomaly found (14.61% ± 5.12%), a relationship between this anomaly and total motility was observed (r = - 0.521; P-value = 0.001). The comparison between the motility results obtained by the subjective method (40.42% ± 15.04%) and the CASA system analysis (49.99% ± 17.62%) revealed a highly significant correlation between both (r = 0.802; P-value <0 , 0001). The results, although not conclusive in their entirety, due not only to the many doubts about CASA, but also due to the small population sample, show that there is a large discrepancy between the subjective motility value and the value of progressive mobile sperm showing that the technician's assessment is much closer to the value of total motility, further research and use of CASA systems in the future of cryopreservation is essential.
Currently, CASA technology has several advantages, yet there is no standardization of specific parameters for each animal species, due to the scarce or incomplete bibliography. The aim of this study was to evaluate the viability of cryopreserved semen from 9 Serpentine bucks through the Computer Assisted Sperm Analysis (CASA). Regarding motility, Total and Progressive were 49.99% ± 17.62% and 13.93% ± 9.04%, respectively. The average velocity values: VCL, VSL and VAP were 76.29%, 37.04%, and 50.03%. Observation of morphology revealed 75% ± 10.48% of normal defrost forms, with tail defects being the most common anomaly found (14.61% ± 5.12%), a relationship between this anomaly and total motility was observed (r = - 0.521; P-value = 0.001). The comparison between the motility results obtained by the subjective method (40.42% ± 15.04%) and the CASA system analysis (49.99% ± 17.62%) revealed a highly significant correlation between both (r = 0.802; P-value <0 , 0001). The results, although not conclusive in their entirety, due not only to the many doubts about CASA, but also due to the small population sample, show that there is a large discrepancy between the subjective motility value and the value of progressive mobile sperm showing that the technician's assessment is much closer to the value of total motility, further research and use of CASA systems in the future of cryopreservation is essential.
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Orientação: Carlos Varela Bettencourt; co-orientação: Daniel de Moura Murta
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MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA, MEDICINA VETERINÁRIA, VETERINÁRIA, VETERINARY MEDICINE, CRIOPRESERVAÇÃO DE SÉMEN, SEMEN CRYOPRESERVATION, CAPRINOS, GOATS