Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10451/38874
Título: | Gymnodinium catenatum Graham isolated from the Portuguese coast: Toxin content and genetic characterization |
Autor: | Silva, Teresa Caeiro, Maria F. Costa, Pedro Reis Amorim, Ana |
Palavras-chave: | Gymnodinium catenatum Intra-specific variability PST profile Strain origin Single nucleotide polymorphism Portugal |
Data: | 2015 |
Resumo: | The bloom forming marine dinoflagellate Gymnodinium catenatum Graham has been linked to paralytic shellfish poisoning (PSP) outbreaks in humans. Along the Portuguese coast (NE Atlantic), G. catenatum shows a complex bloom pattern, raising questions about the origin and affinities of each bloom population. In this work, the variability within six cultured strains of G. catenatum isolated from Portuguese coastal waters (S coast, W coast and NW coast), between 1999 and 2011, was investigated. The strains were analyzed for toxin profiling and intra-specific genetic diversity. Regarding the toxin profile, differences recorded between strains could not be assigned to the time of isolation or geographical origin. The parameter that most influenced the toxin profile was the life-cycle stage that originated the culture: vegetative cell versus hypnozygote (resting cyst). At the genetic level, all strains showed similar sequences for the D1-D2 region of the large subunit (LSU) of the nuclear ribosomal DNA (rDNA) and shared complete identity with strains from Spain, Algeria, China and Australia. Conversely, we did not find a total identity match for the ITS-5.8S nuclear rDNA fragment. After sequence analysis, two guanine/adenine (R) single nucleotide polymorphisms (SNP 1 and 2) were detected for all strains, in the ITS1 region. This species has been reported to present very conservative LSU and ITS-5.8S rDNA regions, though with few SNP, including SNP1 of this study, already attributed to strains from certain locations. The SNP here described characterize G. catenatum populations from Portuguese waters and may represent valuable genetic markers for studies on the phylogeography of this species. |
Peer review: | yes |
URI: | http://hdl.handle.net/10451/38874 |
DOI: | 10.1016/j.hal.2015.07.008 |
ISSN: | 1568-9883 |
Aparece nas colecções: | MARE - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
MARE_ULisboa_HarmfulAlgae_48_2015_94-104.pdf | 1,79 MB | Adobe PDF | Ver/Abrir Acesso Restrito. Solicitar cópia ao autor! |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.