Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10451/50878
Título: | A minimal, unstrained S‐Allyl handle for pre‐targeting Diels–Alder bioorthogonal labeling in live cells |
Autor: | Oliveira, Bruno L. Guo, Zijian Boutureira, Omar Guerreiro, Ana Jiménez‐Osés, Gonzalo Bernardes, Gonçalo J. L. |
Palavras-chave: | Diels-Alder reactions Apoptosis Bioorthogonal labeling Pre-targeting Unstrained alkenes |
Data: | 2016 |
Editora: | Wiley |
Citação: | Angew Chem Int Ed Engl. 2016 Nov 14;55(47):14683-14687 |
Resumo: | The unstrained S-allyl cysteine amino acid was site-specifically installed on apoptosis protein biomarkers and was further used as a chemical handle and ligation partner for 1,2,4,5-tetrazines by means of an inverse-electron-demand Diels-Alder reaction. We demonstrate the utility of this minimal handle for the efficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach. The small size, easy chemical installation, and selective reactivity of the S-allyl handle towards tetrazines should be readily extendable to other proteins and biomolecules, which could facilitate their labeling within live cells. |
Descrição: | © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Peer review: | yes |
URI: | http://hdl.handle.net/10451/50878 |
DOI: | 10.1002/anie.201608438 |
ISSN: | 1433-7851 |
Versão do Editor: | https://onlinelibrary.wiley.com/journal/15213773 |
Aparece nas colecções: | FM - Artigos em Revistas Internacionais IMM - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Minimal_unstrained.pdf | 1,94 MB | Adobe PDF | Ver/Abrir |
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