Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis
Entity
UAM. Departamento de Medicina; Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD)Publisher
BioMed CentralDate
2015-08-27Citation
10.1186/s12891-015-0664-5
BMC Musculoskeletal Disorders 16 (2015): 226
ISSN
1471-2474DOI
10.1186/s12891-015-0664-5Funded by
This work was partially supported by the Arthritis Research UK Pain Centre (Arthritis Research UK, grant number 18769) and by research grant from the Instituto Carlos III (PI13/00570 and PI12/00144). IP-P was funded by an ECTS exchange scholarship grant and an investigator mobility grant from the Autonomous University of Madrid.Editor's Version
http://dx.doi.org/10.1186/s12891-015-0664-5Subjects
Giant cells; Synovitis; Arthritis; MedicinaRights
© 2015 Prieto-Potin et al.Abstract
Background: Multinucleated giant cells have been noticed in diverse arthritic conditions since their first description
in rheumatoid synovium. However, their role in the pathogenesis of osteoarthritis (OA) or rheumatoid arthritis (RA)
still remains broadly unknown. We aimed to study the presence and characteristics of multinucleated giant cells
(MGC) both in synovium and in subchondral bone tissues of patients with OA or RA.
Methods: Knee synovial and subchondral bone samples were from age-matched patients undergoing total joint
replacement for OA or RA, or non-arthritic post mortem (PM) controls. OA synovium was stratified by histological
inflammation grade using index tissue sections. Synovitis was assessed by Krenn score. Histological studies
employed specific antibodies against macrophage markers or cathepsin K, or TRAP enzymatic assay.
Results: Inflamed OA and RA synovia displayed more multinucleated giant cells than did non-inflamed OA and
PM synovia. There was a significant association between MGC numbers and synovitis severity. A TRAP negative/
cathepsin K negative Langhans-like subtype was predominant in OA, whereas both Langhans-like and TRAP-positive/
cathepsin K-negative foreign-body-like subtypes were most commonly detected in RA. Plasma-like and foam-like
subtypes also were observed in OA and RA synovia, and the latter was found surrounding adipocytes. TRAP positive/
cathepsin K positive osteoclasts were only identified adjacent to subchondral bone surfaces. TRAP positive osteoclasts
were significantly increased in subchondral bone in OA and RA compared to PM controls.
Conclusions: Multinucleated giant cells are associated with synovitis severity, and subchondral osteoclast numbers are
increased in OA, as well as in RA. Further research targeting multinucleated giant cells is warranted to elucidate their
contributions to the symptoms and joint damage associated with arthritis
Files in this item
Google™ Scholar:Prieto-Potin, Iván
-
Largo, Raquel
-
Roman-Blas, Jorge A.
-
Herrero-Beaumont Cuenca, Gabriel
-
Walsh, David A.
This item appears in the following Collection(s)
- Producción científica de la UAM [24112]
Related items
Showing items related by title, author, creator and subject.