有鉤囊虫症の血清診断法に関する研究

Abstract

The present study was designed to determine optimum conditions of ELISA for the immunodiagnosis of Cysticercus cellulosae infection in humans and in pigs using four antigens extracted from C. cellulosae (Cyst fluid antigen, Ccf. Ag ; Cyst body antigen, Ccb. Ag ; Cyst wall antigen, Ccw. Ag ; Conus derived antigen, Ccc. Ag). The usefulness of the proposed methods was assessed. The following results were obtained. 1. Ccf. Ag was most useful for the immunodiagnosis of C. cellulosae infection in pigs. Optimum conditions in the ELISA were at the concentration of 15 μg/ml of Ccf. Ag, 10,000 times diluted peroxidase-labelled anti-porcine IgG and at 100 dilution of test sera. More stable results were obtained by blocking the epitope of porcine IgG in Ccf. Ag with rabbit anti-porcine IgG antiserum. 2. In the case of cerebral cysticercosis cellulosae hominis, Ccf. Ag was most sensitive and specific for the serodiagnosis. The optimum conditions, when this antigen was used, were same as used for the infection in pigs. Under these test-conditions by ELISA, significant differences were obtained among cysticercosis and other parasite infections (taeniasis saginata, diphyllobothriasis latum, sparganosis mansoni, diplogonoporiasis grandis, fascioliasis, clonorchiasis, paragonimiasis, strongyloidiasis and ascariasis). 3. When patient sera absorbed with T. saginata antigens was used, much greater differences were obtained among cysticercosis and other parasitic infections with the ELISA using Ccf as an antigen. These results indicate that the ELISA using Ccf as an antigen is most sensitive and specific for screening Cysticercus cellulosae infection.