Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/14922

Fecundity, in vitro early larval development and karyotype of the zoonotic nematode "Anisakis pegreffii"


Vista previa

Ver/Abrir:
 Fecundity_Moratal_VP_2023.pdf
10,86 MB
Adobe PDF
Título : Fecundity, in vitro early larval development and karyotype of the zoonotic nematode "Anisakis pegreffii"
Autor : Moratal, Samantha
Zrzavá, Magda
Hrabar, Jerko
Dea Ayuela, María Auxiliadora
López Ramon, Jordi
Mladineo, Ivona
Materias: Pez marinoMarine fishParasitologíaParasitologyEnfermedad transmisibleInfectious diseasesEnfermedad animalAnimal diseases
Editorial : Elsevier
Citación : Moratal, S., Zrzavá, M., Hrabar, J., Dea-Ayuela, M.A., López-Ramon, J. & Mladineo, I. (2023). Fecundity, in vitro early larval development and karyotype of the zoonotic nematode "Anisakis pegreffii". Veterinary Parasitology, vol. 323 (nov.), art. 110050. DOI: https://doi.org/10.1016/j.vetpar.2023.110050
Resumen : The in vitro life cycle of zoonotic helminths is an essential tool for -omic translational studies focused on disease control and treatment. Anisakiosis is an emerging zoonosis contracted by the ingestion of raw or undercooked fish infected with the third stage larvae (L3) of two sibling species Anisakis simplex sensu stricto (s.s.) and Anisakis pegreffii, the latter being the predominant species in the Mediterranean basin. Recently, in vitro culture of A. pegreffii has been developed to enable fast and large-scale production of fertile adults. However, the conditions for larval development from hatching to infective L3 were not fulfilled to complete the cycle. Herein, we used a Drosophila medium supplemented with chicken serum and adjusted different osmolarities to maintain the culture of L3 hatched from eggs for up to 17 weeks. The highest survival rate was observed in the medium with the highest osmolarities, which also allowed the highest larval exsheathment rate. Key morphological features of embryogenesis and postembryogenesis studied by transmission electron microscopy revealed that the excretory gland cell is differentiated already up to 48 h post-hatching. Extracellular vesicles and cell-free mitochondria are discharged between the two cuticle sheets of the second stage larvae (L2). Contemporarly cultivated, two populations of adult A. simplex s.s. and A. pegreffii reached an average production of 29,914.05 (± 27,629.36) and 24,370.96 (± 12,564.86) eggs/day/female, respectively. The chromosome spreads of A. pegreffii obtained from mature gonads suggests a diploid karyotype formula of 2n = 18. The development of a reliable protocol for the in vitro culture of a polyxenous nematode such as Anisakis spp. will serve to screen for much needed novel drug targets, but also to study the intricated and unknown ecological and physiological traits of these trophically transmitted marine nematodes.
URI : http://hdl.handle.net/10637/14922
Derechos: Open Access
http://creativecommons.org/licenses/by-nc/4.0/deed.es
ISSN : 0304-4017
Cubierto por: Acuerdo Transformativo – 2023
Fecha de publicación : 12-nov-2023
Centro : Universidad Cardenal Herrera-CEU
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.