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Role of RPTPβ/ζ in neuroinflammation and microglia‑neuron communication


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Título : Role of RPTPβ/ζ in neuroinflammation and microglia‑neuron communication
Autor : Fernández Calle, Rosalía
Galán Llario, Milagros
Gramage, Esther
Zapatería Gómez, Begoña
Vicente Rodríguez, Marta
Zapico Rodríguez, José María
Pascual-Teresa Fernández, Beatriz de
Ramos González, Ana
Ramos Álvarez, María del Pilar
Ferrer Alcón, Marcel
Uribarri, María
Herradón Gil-Gallardo, Gonzalo
Materias: NeuroinflammationPleiotrophin
Editorial : Nature Research
Citación : Fernández-Calle R, Galán-Llario M, Gramage E, Zapatería B, Vicente-Rodríguez M, Zapico JM, et al. Role of RPTPβ/ζ in neuroinflammation and microglia-neuron communication [Internet]. Vol. 10, Scientific Reports. 2020.
Resumen : Pleiotrophin (PTN) is a cytokine that is upregulated in different neuroinflammatory disorders. Using mice with transgenic PTN overexpression in the brain (Ptn-Tg), we have found a positive correlation between iNos and Tnfα mRNA and Ptn mRNA levels in the prefrontal cortex (PFC) of LPS-treated mice. PTN is an inhibitor of Receptor Protein Tyrosine Phosphatase (RPTP) β/ζ, which is mainly expressed in the central nervous system. We aimed to test if RPTPβ/ζ is involved in the modulation of neuroinflammatory responses using specific inhibitors of RPTPβ/ζ (MY10 and MY33-3). Treatment with MY10 potentiated LPS-induced microglial responses in the mouse PFC. Surprisingly, MY10 caused a decrease in LPS-induced NF-κB p65 expression, suggesting that RPTPβ/ζ may be involved in a novel mechanism of potentiation of microglial activation independent of the NF-κB p65 pathway. MY33-3 and MY10 limited LPS-induced nitrites production and iNos increases in BV2 microglial cells. SH-SY5Y neuronal cells were treated with the conditioned media from MY10/LPS-treated BV2 cells. Conditioned media from non-stimulated and from LPS-stimulated BV2 cells increased the viability of SH-SY5Y cultures. RPTPβ/ζ inhibition in microglial cells disrupted this neurotrophic effect of microglia, suggesting that RPTPβ/ζ plays a role in the neurotrophic phenotype of microglia and in microglia-neuron communication.
URI : http://hdl.handle.net/10637/15217
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
OpenAccess
ISSN : 2045-2322
Fecha de publicación : 20-nov-2020
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





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