Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/30503
Title: | Electrospinning of bioactive polycaprolactone-gelatin nanofibres with increased pore size for cartilage tissue engineering applications |
Author: | Semitela, Ângela Girão, André F. Fernandes, Carla Ramalho, Gonçalo Bdikin, Igor Completo, António Marques, Paula A.A.P. |
Keywords: | Cartilage tissue engineering Electrospun scaffold Polycaprolactone Gelatin Pore size Electrospraying-generated microparticles |
Issue Date: | 1-Oct-2020 |
Publisher: | Sage |
Abstract: | Polycaprolactone (PCL) electrospun scaffolds have been widely investigated for cartilage repair application. However, their hydrophobicity and small pore size has been known to prevent cell attachment, proliferation and migration. Here, PCL was blended with gelatin (GEL) combining the favorable biological properties of GEL with the good mechanical performance of the former. Also, polyethylene glycol (PEG) particles were introduced during the electrospinning of the polymers blend by simultaneous electrospraying. These particles were subsequently removed resulting in fibrous scaffolds with enlarged pore size. PCL, GEL and PEG scaffolds formulations were developed and extensively structural and biologically characterized. GEL incorporation on the PCL scaffolds led to a considerably improved cell attachment and proliferation. A substantial pore size and interconnectivity increase was obtained, allowing cell infiltration through the porogenic scaffolds. All together these results suggest that this combined approach may provide a potentially clinically viable strategy for cartilage regeneration. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/30503 |
DOI: | 10.1177/0885328220940194 |
ISSN: | 0885-3282 |
Appears in Collections: | TEMA - Artigos DEM - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Author Manuscript - Electrospinning of bioactive polycaprolactone-gelatin nanofibres with increased pore size for cartilage tissue engineering applications.pdf | 1.16 MB | Adobe PDF | View/Open |
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