Study of the ichthyotoxic microalga Heterosigma akashiwo by transcriptional activation of sublethal marker Hsp70b in Transwell co-culture assays
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URI: http://hdl.handle.net/10835/14802
DOI: https://doi.org/10.1371/journal.pone.0201438
DOI: https://doi.org/10.1371/journal.pone.0201438
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Rivera, Alejandra; Vega-Drake, Karina; Aburto, Carla; Cruzat, Fernando; Ulloa, Viviana; [et al.]Fecha
2018-08-02Resumen
Despite the advance of knowledge about the factors and potential mechanisms triggering
the ichthyotoxicity in microalgae, these remain unclear or are controversial for several species
(e.g. Heterosigma). Neither typical toxicity tests carried out with cell extracts nor direct
exposure to harmful species were proved suitable to unravel the mechanism of harm.
Ichthyotoxic species show a complex harmful effect on fish, which is mediated through various
mechanisms depending on the species. In this work, we present a method to study sublethal
effects triggered by reactive oxygen species of a population of harmful algae in vivo
over a fish cell line. To that end, Transwell co-cultures in which causative and target species
are separated by a 0.4 μm pore membrane were carried out. This allowed the evaluation of
the effect of the released molecules by cells in a rapid and compact test. In our method, the
harmful effect was sensed through the transcriptional activation of sub-lethal ma...