Phosphorus fertilization management modifies the biodiversity of AM fungi in a tropical savanna forage system

Abstract

In the present study we investigated how the community of arbuscular mycorrhizal fungi (AMF) in roots of Centrosema macrocarpum responded to different doses and sources of phosphorus (40 kg ha 1 of P as rock phosphate, 150 kg ha 1 of P as rock phosphate and 75 kg ha 1 of P as diammonium phosphate together with 75 kg ha 1 of P as rock phosphate) in a Venezuelan savanna ecosystem. We also related AMF diversity to soil parameters (total N, total P, available P, extractable K, total Ca, total Mg, total Fe, total Cu, total Zn, total Mn, glomalin-related soil protein, microbial biomass C, dehydrogenase, urease and acid phosphatase activities, water-soluble carbon and carbohydrates and aggregate stability) at different doses of P. The AM fungal small-subunit (SSU) rRNA genes were subjected to PCR, cloning, SSCP, sequencing and phylogenetic analyses. Nine fungal types were identified: six belonged to the genus Glomus and three to Acaulospora. The majority of fungal types showed high similarity to sequences of known glomalean isolates: Aca 1 to Acaulospora mellea, Aca 2 to Acaulospora rugosa, Aca 3 to Acaulospora spinosa, Glo 1 to Glomus intraradices and Glo 3 to Glomus fasciculatum. The control treatment was dominated by species belonging to the genus Acaulospora. However, when the soil was fertilised with low doses of P, the colonisation of roots increased and there was a change in the AMF diversity, the genus Glomus dominating. The AM development and the abundance of AM fungal types in roots were decreased dramatically by the fertilisation with high doses of P, without differences between the source of P used. The available P in soil was negatively correlated with the AMF diversity. In conclusion, the application of low doses of P as rock phosphate stimulated mycorrhization and enhanced the soil quality parameters except water- oluble carbohydrates, helping to offset a loss of fertility in P-poor tropical savanna soils.