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Compartmentalization and metabolic channeling for multienzymatic biosynthesis : practical strategies and modeling approaches
Publikationstyp
Journal Article
Date Issued
2013-08-10
Sprache
English
Author(s)
Institut
TORE-URI
Start Page
41
End Page
65
Citation
Fundamentals and application of new bioproduction systems / An-Ping Zeng, ed. With contr. by Inés Ardao ... - Berlin ; Heidelberg [u.a.] : Springer, 2013 . - (Advances in biochemical engineering/biotechnology ; 137). - Seite 41-65
Publisher DOI
Scopus ID
PubMed ID
23934361
Publisher
Springer
The construction of efficient enzyme complexes for multienzymatic biosynthesis is of increasing interest in order to achieve maximum yield and to minimize the interference due to shortcomings that are typical for straightforward one-pot multienzyme catalysis. These include product or intermediate feedback inhibition, degeneration, and diffusive losses of reaction intermediates, consumption of co-factors, and others. The main mechanisms in nature to tackle these effects in transient or stable protein associations are the formation of metabolic channeling and microcompartments, processes that are desirable also for multienzymatic biosynthesis in vitro. This chapter provides an overview over two main aspects. First, numerous recent strategies for establishing compartmentalized multienzyme associations and constructed synthetic enzyme complexes are reviewed. Second, the computational methods at hand to investigate and optimize such associations systematically, especially with focus on large multienzyme complexes and metabolic channeling, are discussed. Perspectives on future studies of multienzymatic biosynthesis concerning compartmentalization and metabolic channeling are presented.
Subjects
Compartmentalization
Metabolic channeling
Modeling
Multienzymatic synthesis
Synthetic biology
DDC Class
570: Biowissenschaften, Biologie
600: Technik
620: Ingenieurwissenschaften